In addition, the symptoms of oxidative stress, inflammation, and hepatic lipid accumulation of liver were ameliorated by DOP administration. The above results suggest that the study of functional ingredient application of DOP might be meaningful for the management of T2D.Cellulose nanofibrils (CNFs) with cellulose I and II allomorphs were efficiently prepared by sulfuric acid hydrolysis of eucalyptus cellulose under three different conditions followed by mechanical treatments (i) classical sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization and hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II). Crystal structure, surface chemistry, morphology and thermal properties of three CNFs were investigated and compared. Three CNFs of 2-10 wt% were mixed with PVA to prepare biodegradable composite films. CNF allomorph had significant impact on crystal structure, mechanical and thermal properties of the PVA composites. The maximum Young’s modulus of CNF-I/PVA, RNF-II/PVA and MNF-II/PVA were increased by 62, 32 and 44%, respectively. Addition of CNFs raised onset degradation temperature (Tonset) and thermal decomposition temperature (Tmax) of PVA nanocomposite, while decreased the melting temperature (Tm). Temperature window (Tonset – Tm) for the melt processing of nanocomposites (10 wt% CNF loading) were increased 2.8, 3.2 and 2.5 times for CNF-I/PVA, RNF-II/PVA and MNF-II/PVA, respectively. All composite films remained excellent transparency with addition of CNFs. This comparative study provided important knowledge of selecting CNF allomorph for fabrication of high-performance CNF/PVA composites.Severe side effects and the rapid emergence of drug resistance in cancer cells are major problems in the chemotherapy utilizing anthracyclines, with a difference between cellular response at nano and micro scale levels. Understanding this situation is more complicated issue to attain efficient targeted formulations with low unexpected toxicity in patients. On nano-scale level, considering properties of nano-bio interaction in all relevant parts of the body may offer clue for suitable formulations. Four main strategies comprising PEGylation, surface charging, targeting, and stimuli responsiveness can be deployed to improve the liposomal and polymeric nanoformulations that can efficiently deliver common anthracyclines namely daunorubicin (DAU), doxorubicin (DOX), idarubicin (IDA), and epirubicin (EPI). Herein, the advances and challenges pertaining to the formulations of these anticancer drugs via liposomal and polymeric nanoformulations, are discussed.Green agrochemicals are important guarantee for food production and security, and target protein identification is the most important basis for development of novel agrochemicals. Affinity chromatography methods for immobilization of agrochemicals have been widely used to identify and confirm new targets. However, this method often requires modification of the active molecules which can affect or damage its biological activity, and biomacromolecules, particularly most natural products, are hard to be modified either. In order to overcome the shortcomings of molecular modification, label-free technology has been developed based on evaluating responses to thermal or proteolytic treatments. Combined with the chemical biology technology and molecular biology technology, it has been used in the development of drugs and agrochemicals. Herein, common methods of label-free technology for identification of direct target of agrochemicals are reviewed, including the principle, advantages, limitations and applications in the research of agrochemicals in the last decade. And the methods for validation of candidate targets obtained by the label-free methods are also reviewed, which are important to obtain the accurate and reliable targets. Combined application of these methods will greatly reduce the experimental costs and shorten the period for the new target identification and validation by improving its accuracy, which will provide a systematic solution for new ecological agrochemicals research and development.Bio scaffolds used for cutaneous tissue regeneration is a challenging issue in the healthcare system. To help this problem, we aimed to report on fabrication and characterization of citric acid cross-linked carboxymethyl guar gum (CMGG) nanocomposite films loaded with ciprofloxacin for faster wound healing application. Differential scanning calorimeter (DSC) and attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy studies, dynamic light scattering (DLS), scanning electron microscopy (SEM) and X-ray diffraction (XRD) were used as analytical techniques for characterization of the nanocomposite film. The morphological characters of nanocomposite film were determined by SEM. The prepared scaffolds were evaluated for the carboxyl content and swelling ratio. Ciprofloxacin was loaded into scaffold and drug release was studied at pH 7.4. The hemolysis assay was used to study the biocompatibility of scaffold films. selleck chemicals The formation of ester cross-links between citric acid and CMGG was confirmed by DSC and ATR- FTIR. The total carboxyl content of scaffold was found to be decreased when the amount of CMGG was increased. The swelling of scaffold film was found to be decreased with increase in curing temperature and time. CMGG scaffold films showed high drug loading with non-Fickian release mechanism suggesting controlled release of drug. In vivo wound healing studies were carried out for 5 days. In this study we observed a faster wound healing effect within 5 days by incorporation of ciprofloxacin in the CMGG film and found biocompatible. Hence, these Nanocomposite films show greater potential in treating wounds.Flavobacteriia are important degraders in the marine carbon cycle, due to their ability to efficiently degrade complex algal polysaccharides. A novel exo-(α-1,3)-3,6-anhydro-D-galactosidase activity was recently discovered from a marine Flavobacteriia (Zobellia galactanivorans DsijT) on red algal carrageenan oligosaccharides. The enzyme activity is encoded by a gene found in the first described carrageenan-specific polysaccharide utilization locus (CarPUL) that codes for a family 129 glycoside hydrolase (GH129). The GH129 family is a CAZy family that is strictly partitioned into two niche-based clades clade 1 contains human host bacterial enzymes and clade 2 contains marine bacterial enzymes. Clade 2 includes the GH129 exo-(α-1,3)-3,6-anhydro-D-galactosidase from Z. galactanivorans (ZgGH129). Despite the discovery of the unique activity for ZgGH129, finer details on the natural substrate specificity for this enzyme are lacking. Examination of enzyme activity on natural carrageenan oligomers using mass spectrometry demonstrated that ZgGH129 hydrolyses terminal 3,6-anhydro-D-galactose from unsulfated non-reducing end neo-β-carrabiose motifs.