Mixed corticomedullary tumor is an adrenal tumor intermixed with cortical and medullary cells. It is extremely rare with unclear tumorigenesis. We reported a 32-year-old female, manifested with typical Cushing’s syndrome and hypertension, to be diagnosed with right huge adrenal mixed corticomedullary tumor (8.8 cm). Right adrenalectomy was done to document the tumor intimately admixed with adrenal cortical adenoma and pheochromocytoma by biochemistry and immunohistochemistry. A case-control study was designed to explore the tumorigenesis of mixed corticomedullary tumor by whole exome sequencing. Expression of the stemness markers was controlled by a tissue array of 80 adrenal tumors. Overall, 1559 identical variants coexisted in parts of adrenal cortical adenoma and pheochromocytoma, which mainly (85.8%) originated from germline mutations. These enriched mutations were engaged in stemness control, coherent with substantial expression of the stemness markers (SOX2, CD44 and OCT4) in both parts. The differential stemness expressions were demonstrated in other adrenal tumors as well. The germline mutations were also enriched in signaling involving cancer proliferation, hypoxia inducible factor-1, focal adhesion and extracellular matrix receptor interaction. Somatic mutations affecting mitogen-activated protein kinase signaling, glycolysis and the citrate cycle were found in some tumor elements. This is the first study to verify the rare mixed corticomedullary tumor by molecular and genetic evidence to link with its phenotype. Germline mutations involving the stemness regulation and cancer proliferative signaling may drive intermixed tumor formation. Somatic mutations related to glycolysis and the citrate cycle may contribute to greater tumor outgrowth.Rotational spectra of 2-methoxypyridine and its monohydrate have been characterized by pulsed jet Fourier transform microwave spectroscopy and complementary ab initio calculations. Rotational spectra of the parent monomer and seven mono-substituted isotopologues (13C and 15N) were measured in natural abundance, which allow determining the accurate structure of the skeleton of 2-methyoxypyridine. The barrier to the methyl internal rotation was determined from the A/E torsional symmetry species of the rotational transitions. For the 2-methyoxypyridine⋯H2O complex, rotational spectra of the parent dimer and isotopologues formed with isotopically enriched water (H218O, HOD, DOH and D2O) indicate that the observed isomer is stabilized by a strong OH⋯N and a secondary bifurcated (CH)2⋯O weak hydrogen bonds. Bader’s quantum theory of atoms in molecules and Johnson’s non-covalent interaction analyses were applied and visualized to have a better understanding of the non-covalent interactions in 2-methyoxypyridine⋯H2O.Solvents as complex environments can induce considerable effects on the physical, chemical and biological properties of solute molecules. In this work, solvent polarity sensitive characteristics of two tautomers of different azo compounds were studied by using of spectroscopic and Z-scan techniques. In this case, nature and various molecular interactions contribution on the spectral behaviors of azo and hydrazone forms of selected samples were investigated by Kamlet-Abboud-Taft and Catalan models. Moreover, the obtained results of Z-scan indicate that self-defocusing, saturable and reverse saturable absorption features of azo dyes depend highly on substituents and molecular environment polarity characteristic. In this case, media polarity properties have the same effects on the third order nonlinear absorption coefficient and nonlinear refractive index values. BAY-3827 supplier Therefore, by measuring one of the third order nonlinear optical coefficients, it is possible to predict solvent polarity effects on the other nonlinear optical coefficient. The calculated dipole moments with high values in the molecular excited state also reveal the probability of molecular charge transfer characteristics. In this case, by excitation process, sample I indicate high dipole moment values in comparison to other samples. Therefore, the results can give useful information about the application of selected samples for designing various optical devices.A metal-organic semiconductor-molecule model was developed with Ag nanoparticles (NPs), poly(3,4-ethylenedioxythiophene)poly(styrenesulfonate) (PEDOTPSS) and 4-mercaptobenzoic acid (4-MBA) via the layer-by-layer self-assembly method. In the SERS spectrum of the Ag/PEDOTPSS/4-MBA system, structural changes in the PEDOT chain were discovered, which provides a deeper understanding of the charge transfer (CT) mechanism in SERS and helps in the development of a method to construct metal-organic semiconductor SERS substrates. A quantitative calculation of the degree of charge transfer (ρCT(κ)) determines the CT contribution of PEDOTPSS to the SERS intensity of the Ag/PEDOTPSS/4-MBA system. On this basis, we propose the formation of a resonance complex between Ag NPs and PEDOTPSS to explore the CT mechanism, which is beneficial for studying interface CT and for understanding the CT mechanism in SERS. The introduction of organic semiconductors in this study not only broadens the research scope of SERS substrates but also contributes to the exploration of SERS mechanisms.Objectives Recommended perioperative nutritional interventions may contribute to satisfactory surgical outcomes. Each moment in the course of a surgical pathologic condition may be a window of opportunity for an intervention with a positive impact on postoperative recovery. Based on the idea of accelerating postoperative recovery, the objective of this study was to evaluate the effectiveness of a nutritional intervention with preoperative fasting abbreviation (Enhanced Recovery After Surgery recommendations) on the metabolism of patients undergoing gynecologic surgeries under spinal anesthesia. Methods This randomized clinical trial was performed at a hospital of medium complexity. After Human Research Ethics Committee approval on August 24, 2015, 80 women who had gynecologic surgery in the period from January to June 2016 and signed the consent form were randomly allocated into two groups a control group (n = 42) and a juice group (n = 38). They received 200 mL of inert solution (control group) or liquid enriched with carbohydrate and protein (juice group) 4 h before surgery.