To our knowledge, this is the first report of isolation and characterization of Photobacterium damselae subsp. damselae from natural diseased P. vannamei. Our findings can serve as a basis for further studies of its pathogenicity and provide technological support for disease controlling in shrimp aquaculture. © 2020 John Wiley & Sons Ltd.OBJECTIVES Metabolic reprogramming is well accepted as a hallmark of cancer. This study aimed to explore the role of Kruppel-like factor 2 (KLF2) in aerobic glycolysis and glutamine consumption of energy metabolism in non-small cell lung cancer (NSCLC) cells. METHODS Two different NSCLC cells, A549 and NCI-H1299, were used to investigate the role of KLF2 in glycolysis and glutamine consumption by tracer technique and KLF2 transfection. KEY FINDINGS The results showed that overexpression KLF could inhibit the energy metabolism and proliferation of NSCLC cells, but had no significant effect on glycolysis reaction and only affected the glutamine consumption of NSCLC cells. In NSCLC cells exposed to glutamine deprivation, the effect of overexpression of KLF2 on cell proliferation and energy metabolism disappeared. It was found that KLF2 could inhibit the expression of glutaminase (GLS) by metabolite tracing technique and so on. However, when GLS inhibitors were given to overexpressing KLF2 NSCLC cells, the intervention effect of KLF2 disappeared. CONCLUSIONS Kruppel-like factor 2 could decrease the level of glutamine, participate in the consumption of glutamine by cancer cells, and then inhibit the energy metabolism of cancer cells. © 2020 Royal Pharmaceutical Society.in English, French INTRODUCTION Les propriétés sensorielles ont une grande importance pour les cosmétiques et les produits de soins. Si la littérature permet de consulter des articles comparant différentes formulations sur leurs attributs sensoriels, il n’y a que quelques articles concernant les ingrédients. OBJECTIF L’objectif global de cette étude était de réaliser une étude originale combinant l’évaluation sensorielle de différentes natures d’ingrédients, afin d’initier un ensemble de données sensorielles qui pourrait aider les chercheurs à identifier les caractéristiques sensorielles discriminantes, ainsi qu’à initier une comparaison entre données sensorielles et mesures instrumentales du texturomètre. MÉTHODES Les huiles, les extraits glycoliques, le beurre et l’amidon ont été évalués selon la méthodologie d’analyse sensorielle descriptive avec l’aide d’un panel de 25 experts. Afin de comparer les réponses obtenues à partir du panel aux mesures instrumentales, des analyses de texture ont été effectuées avec un texturométre selon la méthode traction-compression. RÉSULTATS Des différences significatives ont été détectées sur dix des onze attributs sensoriels évalués, indiquant que les ingrédients ont des profils sensoriels distincts. Les attributs principalement discriminants pour les ingrédients analysés étaient la brillance, l’opacité, la fluidité, la fraîcheur, le blanchiment et les résidus huileux. Les huiles sont principalement liées aux descripteurs résidus huileux et glissant tandis que les extraits sont principalement liés aux attributs de brillance, de fluidité et de fraîcheur. L’amidon de tapioca et le beurre de karité étaient tous deux liés à la non-fluidité, l’opacité et l’amidon de tapioca était également lié à la fraîcheur. VX-680 ic50 Cette étude a été complétée par une analyse texturométrique qui a montré la corrélation opposée entre le descripteur sensoriel fluide et l’indice de consistance.Tremendous strides have been made in the identification of microbes (animalcules) since their first discovery on epithelial surfaces by Antonie van Leeuwenhoek in 1677. For example, Staphylococcus aureus, a bacterium associated with atopic dermatitis, was identified by Friedrich Rosenbach in 1884 and Propionibacterium acnes, now called Cutibacterium acnes – a major commensal bacterium found primarily on the face – by Raymond Sabouraud in 1897. This article is protected by copyright. All rights reserved.Intelligent-design websites misquote to subvert belief in Darwinian evolution. Nowadays, such sites pose as “objective” sources of information. Speaking more generally, spreading misinformation can be linked to climate science denial, vaccination avoidance, and a resurgence of pseudo-scientific racism. Internet regulations to counter these sources of pseudo-science are urgently needed. © 2020 The Authors. BioEssays published by Wiley Periodicals LLC.BACKGROUND Blood transfusion is common and potentially lifesaving but is associated with risk and overuse. Nurse practitioners (NPs) in multidisciplinary care teams are increasingly expanding their scope of practice to transfusion medicine (TM). Resources aimed at NPs are lacking, and little is known about NP TM knowledge. Thus, we developed a pilot TM curriculum for NP credentialing and assessed its impact. METHODS NP leads and TM directors adapted the successful Canadian Transfusion Camp for medical postgraduate trainees into a 3-day curriculum for NPs. Two modalities were used to assess the pilot 1) a participant demographics survey and needs assessment; and 2) the validated BEST-TEST knowledge assessment exam administered before and after the course. RESULTS Of the 23 volunteer participants, the majority reported prescribing blood products within the last year, primarily red blood cells. Minimal opportunities to undertake continuing medical education in TM were identified. NPs often used preprinted order forms, consultation with physicians sharing care, or local fact sheets to guide transfusion; rather than TM physician consultation or guidelines. Exam scores significantly improved after the course (before, 35.2% vs. after, 50.3%; p = 0.005), suggesting average initial knowledge being below medical postgraduate trainee-level improving to postgraduate trainee level. Questions on appropriate transfusion triggers and correct recipient identification were most correctly answered; and responses to transfusion reaction questions required improvement. CONCLUSIONS Our needs assessment suggests that TM resources for NPs are relevant but lacking. Our initiative supports the generalizability, scalability, and effectiveness of the Transfusion Camp program. Further implementation, refinement, and future impact assessments are required. © 2020 AABB.