A safe and effective vaccine is urgently needed to control the unprecedented COVID-19 pandemic. Four adenovirus-vectored vaccines expressing spike (S) protein have been approved for use. Here, we generated several recombinant chimpanzee adenovirus (AdC7) vaccines expressing S, receptor-binding domain (RBD), or tandem-repeat dimeric RBD (RBD-tr2). We found vaccination via either intramuscular or intranasal route was highly immunogenic in mice to elicit both humoral and cellular immune responses. AdC7-RBD-tr2 showed higher antibody responses compared to either AdC7-S or AdC7-RBD. see more Intranasal administration of AdC7-RBD-tr2 additionally induced mucosal immunity with neutralizing activity in bronchoalveolar lavage fluid. Either single-dose or two-dose mucosal administration of AdC7-RBD-tr2 protected mice against SARS-CoV-2 challenge, with undetectable subgenomic RNA in lung and relieved lung injury. AdC7-RBD-tr2-elicted sera preserved the neutralizing activity against the circulating variants, especially the Delta variant. These results support AdC7-RBD-tr2 as a promising COVID-19 vaccine candidate.

There is limited information about Turkish children’s mental health during the COVID-19 outbreak. Here, in a Turkish community sample, we investigate the psychological symptoms in primary school children after the start of the COVID-19 outbreak according to caregivers and the caregivers’ perceived stress levels.

The sample was randomly selected from the primary schools in Denizli, Turkey. Denizli is a large city and the total population is around one-million. The study was conducted online. The caregivers responded to a sociodemographic and daily life during pandemic questionnaire and psychological symptoms screening form (which evaluated whether the children had any newly developed symptoms after the start of the COVID-19 outbreak) and the Perceived Stress Scale (PSS). One-thousand-seven-hundred-ninety-seven people were included in the study. Data was collected between 3 July and 31 August 2020.

Symptoms such as anhedonia, gets angry easily, fidgets around, sleeping problems, impulsivity, appetite problems, restlessness or nervousness, and feels sad can be seen more frequently than other symptoms in children. Caregivers’ PSS scores were higher in mothers than fathers. An association was found between the PSS scores of the caregivers and all psychological symptoms questioned in the study for children, except for encopresis. Lower education level of the father, lower family income, having a child with a psychiatric disease, impaired sibling relationship, and impaired functionality at home were risk factors for higher caregivers’ PSS scores.

It may be beneficial to take supportive measures in terms of protecting children’s mental health.

It may be beneficial to take supportive measures in terms of protecting children’s mental health.Today, the use of nutrients derived from natural bioactive compounds application in the food, pharmaceutical, and cosmetic industries is on the increase. This paper aimed to evaluate the amino acids profile (essential and non-essential) and pigments composition (chlorophyll a, carotenoids, and phycocyanin) of Arthrospira platensis (a blue-green microalga) cultivation in a flat-plate photobioreactor under various types of light-emitting diodes (red 620-680 nm, white 380-780 nm, yellow 570-600nm, blue 445-480 nm). The maximum biomass concentration (604.96 mg L-1) occurred when the red LED was applied for cultivation, and the minimum biomass concentration (279.39 mg L-1) was obtained under blue LED. The sequence of pigments and amino acids concentrations (mg L-1culture volume) was approximately in accordance with the biomass productivity. It means the red light produces the maximum concentration of pigments (chlorophyll a 5.42, carotenoids 2.92, phycocyanin 67.54 mg L-1) and amino acids (essential amino acids 110.47, nonessential amino acids 179.10 mg L-1). Nevertheless, when these values were measured in mg per g of dry weight, the utmost contents were observed in microalgal products cultivated under blue LED. These consequences are due to the highest cell productivity and the most extended length of cells that occurred under red and blue LEDs, respectively.

To develop a protocol and a clinical tool to assess the audiological needs of younger and older adults with hearing loss.

A needs assessment protocol was developed based on recommendations of an expert panel, existing literature, the International Classification of Functioning, Disability and Health, client-centered care and goal setting. The protocol was reviewed by the expert panel. Semi-structured interviews were conducted with clinical audiologists to validate its content and explore its clinical applicability. A clinical tool was developed to make the administration of the protocol more uniform.

15 experts and 14 clinical audiologists.

Feedback received from the participants (experts and clinicians) supported the content validity of the needs assessment protocol and clinical tool. The topics covered within the protocol and tool include Audiological needs (activity limitations, participation restrictions, environmental factors), Living conditions (social networks, living environment), Personal factors, Discussion with the client to define the intervention plan, and Recommendations.

A protocol and a clinical tool were developed to help audiologists and clients undertake a comprehensive audiological needs assessment. The content validity of the protocol and tool were demonstrated. Their use can facilitate the delivery of a client-centered assessment using a uniform and comprehensive approach.

A protocol and a clinical tool were developed to help audiologists and clients undertake a comprehensive audiological needs assessment. The content validity of the protocol and tool were demonstrated. Their use can facilitate the delivery of a client-centered assessment using a uniform and comprehensive approach.In this study, an attempt has been made to develop an automated muscle fatigue detection system using cyclostationary based geometric features of surface electromyography (sEMG) signals. For this purpose, signals are acquired from fifty-eight healthy volunteers under dynamic muscle fatiguing contractions. The sEMG signals are preprocessed and the epochs of signals under nonfatigue and fatigue conditions are considered for the analysis. A computationally effective Fast Fourier transform based accumulation algorithm is adapted to compute the spectral correlation density coefficients. The boundary of spectral density coefficients in the complex plane is obtained using alpha shape method. The geometric features, namely, perimeter, area, circularity, bending energy, eccentricity and inertia are extracted from the shape and the machine learning models based on multilayer perceptron (MLP) and extreme learning machine (ELM) are developed using these biomarkers. The results show that the cyclostationarity increases in fatigue condition.