The current pandemic of the 2019 novel coronavirus (COVID-19) caused by SARS-CoV-2 (severe acute respiratory syndrome coronavirus-2) has raised significant public health concern. Rapid, affordable, and accurate diagnostics of SARS-CoV-2 is essential for early treatment and control of the disease spread. In the past few years, CRISPR technology has shown great potential for highly sensitive and specific molecular diagnostics. Amid the ongoing COVID-19 pandemic, there is an increasing interest in implementing CRISPR-based diagnostic principles to develop fast and precise methods for detecting SARS-CoV-2. In this work, we reviewed and summarized these CRISPR-based diagnostic systems as well as their characteristics and challenges. We also provided future perspectives of CRISPR-based sensing towards point-of-care molecular diagnosis applications.The nonspecific adsorption and accumulation of biomolecules on electrode interfaces remains a challenge for sensitive and accurate detection of disease markers in complex biological media, and it is highly desired to develop antifouling biosensors capable of assaying targets in complicated real liquids. Herein, an efficient and simple antifouling biosensor was constructed based on a self-designed Y-shaped peptide. The Y-shaped peptide was designed with two branches one branch with the peptide sequence of EKEKEKE for antifouling, and the other branch with the peptide sequence of HWRGWVA for recognizing of human IgG. Under optimized experimental conditions, electrodes modified with Y-shaped peptides exhibited excellent antifouling and electrochemical sensing performances. The developed biosensor was able to effectively resist biofouling in various protein solutions and even serum samples, and the linear range of the biosensor for human IgG detection was from 100 pg mL-1 to 10 μg mL-1, with a relatively low limit of detection of 32 pg mL-1 (S/N = 3). The antifouling biosensor possesses the capability of assaying human IgG in real serum samples, and this strategy of developing low fouling biosensors based on Y-shaped peptides can be readily expanded to the construction of other biosensing systems for different targets.The outbreak of life-threatening pandemic like COVID-19 necessitated the development of novel, rapid and cost-effective techniques that facilitate detection of viruses like SARS-CoV-2. The presently popular approach of a collection of samples using the nasopharyngeal swab method and subsequent detection of RNA using the real-time polymerase chain reaction suffers from false-positive results and a longer diagnostic time scale. Alternatively, various optical techniques namely optical sensing, spectroscopy, and imaging shows a great promise in virus detection. Herein, a comprehensive review of the various photonics technologies employed for virus detection, particularly the SARS-CoV family, is discussed. The state-of-art research activities in utilizing the photonics tools such as near-infrared spectroscopy, Fourier transform infrared spectroscopy, Raman spectroscopy, fluorescence-based techniques, super-resolution microscopy, surface plasmon resonance-based detection, for virus detection accounted extensively with an emphasis on coronavirus detection. Further, an account of emerging photonics technologies of SARS-CoV-2 detection and future possibilities is also explained. The progress in the field of optical techniques for virus detection unambiguously show a great promise in the development of rapid photonics-based devices for COVID-19 detection.

Piacenza is the closest city to the first coronavirus disease 2019 (COVID-19) cluster in Italy and has the highest national COVID-19 death rates per population. The objective of this study is to present characteristics and outcomes of patients admitted to medical departments of the Hospital of Piacenza during the first wave of the epidemic.

A total of 218 patients with confirmed or suspect COVID-19 and severe pneumonia were included from February 21st to May 15th, 2020. ABT-263 price Routinely-collected clinical and laboratory data were retrospectively retrieved from electronic medical files. A Cox proportional-hazards model was fit to assess the association of treatment and other variables with death.

Median age of patients was 68 years; 150 patients (69%) had comorbidities, mainly hypertension (107, 49%). Overall, 185 (85%) patients had acute respiratory distress syndrome (ARDS) on admission, including 103 (47%) with moderate or severe ARDS. Chest computed tomography scan showed bilateral disease in 201 (98%) and ee of antivirals was not associated with increased survival.Anxiety, perceived control and trust in information sources have all been shown to significantly influence health and social behaviours during pandemics. We measured these factors in a nationally representative on-street survey collected across five regions of Thailand (N = 1000, May 2020, response rate 82.6%). Anxiety was positively associated with stocking up on food (OR 2.62 (95% CI 1.88-3.66)) and taking vitamins (OR 2.37 (1.59-3.54)); perceived control with (recommended) coughing into an elbow (OR 2.42 (1.80-3.26)), checking on others (OR 1.52 (1.00-2.31)), and negatively with stockpiling (OR 0.72 (0.55-0.96)). Those relying on family/friends, doctors online or foreign sources were more likely to take vitamins (ORs 4.11, 2.88. 2.82), respondents using TV news less likely to stock up on food (OR 0.57 (0.37-0.86)) and to wear a mask for self-protection (OR 0.27 (0.10-0.73)). Comparing findings with analogous cross-sectional data on anxiety collected at the start of the pandemic (Feb 2020, Goodwin et al., 2020) there was no significant difference between personal anxiety in the two surveys (F (1, 1197) = 0.72, p = .40)) but perceived control was lower in the later survey (F (1, 1197) = 6.72 p = .01)). Findings suggest reduced perceived control as the pandemic developed and illuminate possible negative impacts of anxiety and low sense of control on pandemic behaviours.Mindfulness-based interventions can enhance cognitive abilities among older adults, thereby effectively delaying cognitive decline. These cognitive enhancements are theorized to accompany neuroplastic changes in the brain. However, this mindfulness-associated neuroplasticity has yet to be documented adequately. A randomized controlled trial was carried out among participants with mild cognitive impairment (MCI) to examine the effects of a mindfulness-based intervention on various cognitive outcomes and cortical thickness (CT) in the context of age-related cognitive impairment. Participants were assigned to a mindfulness awareness program (MAP)(n = 27) and an active control condition – health education program (n = 27). In both, they attended weekly sessions for three months and subsequently, monthly sessions for six months. Cognitive assessments and structural scans were carried out across three time-points. Whole brain analyses on CT were carried out and were supplemented with region of interest-based analyses.