A bisphenol based fluorescence “turn-on” chemosensor 4,4′-(propane-2,2-diyl)bis(2-((E)-(2-(benzo[d]thiazol-2-yl)hydrazineyldene)methyl)phenol) (BHMP) has been synthesized and its sensing behavior was tested towards various ionic species. The chemo-sensing behavior of BHMP has been established through absorption, fluorescence, NMR, and mass spectroscopic techniques. The probe BHMP selectively detects zinc ions over other metal ions and the resulting BHMP + Zn2+ ensemble serves as a secondary probe for the detection of pyrophosphate (PPi) anion specifically over other anions. The spectroscopic studies reveal the fluorescence enhancement of BHMP in association with Zn2+ ions was quenched in the presence of pyrophosphate (PPi) anions. A probable mechanism of this selective sensing behavior was described on the basis of “OFF-ON-OFF” strategy for detection of both cations and anions. Moreover, the biological applicability of the chemosensor BHMP was examined via cell imaging studies.Herein, the presented manuscript provides for an extensive spectrofluorimetric method for micro determination of silver ion. This established method based on the use of the three synthesized 2,6-disubstituted pyridine derivatives (R1, R2 and R3) through exploiting their high fluorescence emission property. A noticeable effect on the fluorescence emission of the reagents after chelation with Ag (I) was monitored. Its noteworthy that the sensitivity and stability of this method was increased by using micellar medium. After chelation with Ag(I), the fluorescence emission of the ligands R1 and R2 were effectively quenched in a regular manner by increasing Ag(I) concentration. In contrast, an increase of the fluorescence intensity for reagent R3 after addition of Ag (I) was observed. The solvatochromism for all reagents under investigation was examined in different solvent. Furthermore, the chelation between Ag(I) and the and designed pyridine reagents was assessed spectrophotometrically. The optimum conditions for the most stable complexes which give a high signal difference were explored and well-determined. The linear range for determination of silver ion were determined and found to be 0.18-1.16, 0.06-0.59 and 0.18-1.43 μg mL-1 for R1, R2 and R3, respectively. The statistical analytical parameters such as LOD, LOQ, SD of slope, SD of intercept and RDS were calculated. In addition, the developed methods were efficaciously applied for determination of Ag(I) in some water samples. These selective complexation methods found to be in good precision compared to official and reported method as revealed F-test.Aerobic granular sludge (AGS) is a type of biofilm with good sedimentation and density, high biomass, high organic load tolerance and toxicity resistance. Oxytetracycline (OTC) is an antibiotic widely used in livestock and aquaculture, and its low absorption and high residue bring many risks and harms to the ecological environment. In this study, an OTC-degrading strain TJ3 was isolated from AGS and identified as Pandoraea sp. The biodegradation characteristics of OTC by strain TJ3 under different environmental conditions were also investigated. The results showed that the optimal initial pH value and temperature for the culture strain were 6.0 and 30 °C, respectively. At an inoculation dose of 6% (v/v), the removal rate of OTC by strain TJ3 was remarkable (59.4%). Furthermore, when the sodium acetate was present as an additional substrate, the biomass and the OTC removal rate of strain TJ3 were improved. The biodegradability of strain TJ3 to OTC was proved by LC-QTOF/MS, and two possible biotransformation products, i.e. m/z 416 and 219, were identified. In the bioaugmentation experiments of AGS by strain TJ3, the average OTC removal rate was 92.89% after the stable operation of bioreactor. The chemical oxygen demand (COD), ammonium nitrogen (NH4+-N) and total phosphorus (TP) were efficiently removed. The microbial community structure had significantly changed at the genus level, and the relative abundance of Zoogloea, Pandoraea, Cloacibacterium and Desulfovibrio increased evidently. These results implied that the OTC removal performance and the structural stability of AGS were improved. In this study, Pandoraea sp. TJ3 was applied to removal OTC for the first time, and results showed that Pandoraea sp. Foxy-5 TJ3 may be a new auxiliary bacterial resource for the biodegradation of OTC and a potential candidate in the treatment of antibiotic wastewater.

Biogenic nanoparticles are gaining attention due to their low toxicity and numerous biomedical applications. Present study aimed to compare the potential anticancer activity of two biogenic silver nanoparticles (bAgNPs and pAgNPs) against human cervical cancer cell lines (HeLa).

bAgNPs were synthesized using Acinetobacter sp. whereas pAgNPs were synthesized using aqueous root extract of Curcuma aromatica. Effect of these nanoparticles on HeLa cells viability was studied using MTT assay and colony formation assay. Anticancer potential was determined using fluorescence microscopy and flow cytometry studies. Bio-compatibility studies were performed against peripheral blood mononuclear cells (PBMCs).

Both the nanoparticles showed 50 % viability of peripheral blood mononuclear cells (PBMCs) when used at high concentration (200 μg/mL). IC

for bAgNPs and pAgNPs against HeLa cells were 17.4 and 14 μg/mL respectively. Colony formation ability of Hela cells was reduced on treatment with both nanoparticles. Acridine orange and ethidium bromide staining demonstrated that bAgNPs were cytostatic whereas pAgNPs were apoptotic. JC-1 dye staining revealed that the mitochondrial membrane potential was affected on treatment with pAgNPs while it remained unchanged on bAgNPs treatment. Flow cytometry confirmed cell cycle arrest in HeLa cells on treatment with nanoparticles further leading to apoptosis in case of pAgNPs. About 77 and 58 % HeLa cells were found in subG1 phase on treatment with bAgNPs and pAgNPs respectively. bAgNPs showed cytostatic effect on HeLa cells arresting the cell growth in subG1 phase, whereas, pAgNPs triggered death of HeLa cells through mitochondrial membrane potential impairment and apoptosis.

Overall, bAgNPs and pAgNPs could be safe and showed potential to be used as anticancer nano-antibiotics against human cervical cancer cells.

Overall, bAgNPs and pAgNPs could be safe and showed potential to be used as anticancer nano-antibiotics against human cervical cancer cells.