Knockdown of PCAT1 suppressed the proliferation and activity, as well as ALP and other DP markers of DP cells by targeting miR-329. Puromycin aminonucleoside Knockdown of PCAT1 regulated miR-329/Wnt10b axis to attenuate β-catenin expression and nucleus translocation to inhibit Wnt/β-catenin signaling. Furthermore, knockdown of PCAT1 suppressed DP sphere induced follicle regeneration and hair growth in nude mice. CONCLUSION PCAT1 maintains characteristics of DP cells by targeting miR-329 to activating Wnt/β-catenin signaling pathway, thereby promoting hair follicle regeneration. Over the past decades, the extensive use of pyrethroids insecticides for vector control has resulted in the development of insecticide resistance. Cytochrome P450 has been recognized to play a critical role in the metabolic detoxification of insecticides. In the current study, Culex pipiens mosquitoes were collected from Giza Governorate in Egypt and tested for insecticide susceptibility against deltamethrin. First detection of Knockdown resistance gene (Kdr) mutations in field collected mosquitoes was performed. Activities of cytochrome oxidase P450 detoxification enzyme that synchronized with the resistance development, was assessed. Expression profiles of cytochrome P450s and their putative corresponding regulating miRNAs, which was previously reported in Cx. pipiens pallens were evaluated in pyrethroid resistant field-collected Cx. pipiens using RT-qPCR and stem-loop RT-qPCR, respectively. Specific stem-loop reverse transcription primers and forward primers were designed for miRNAs profiling. Our results elucidated the pyrethroid resistance development and revealed its relation to the metabolic and target site modification mechanisms with a first report of L1014F-kdr mutation detection. RT-qPCR results have showed an up-regulation in the expression of the studied P450 transcripts. Negative correlations were found between the expression of P450s and their regulatory miRNAs except for CYP9J35, where positive correlation was found with its corresponding miR-13. Interestingly, our data was the first to detect negative correlation between miR-285 and its putative CYP6Cp1 target gene. These findings highlighted the significance of identifying P450 gene along with regulatory miRNAs as a key mechanism implicated in pyrethroid resistance in field Culex vector population. The elucidation of this mechanism would shed light on the development of insecticide resistance and would help in shaping strategies to combat such vectors. PURPOSE Adverse childhood experiences (ACEs) have been shown to be associated with increased risk of mortality. The biobehavioral mechanisms linking adverse events and survival in cancer patients remain unclear. The aims of the study were to (1) examine the rates and types of early adverse events in patients diagnosed with cancer; (2) investigate the association of adverse events with circulating cytokines, representing immune status of the patient; and (3) test whether immune markers mediated the association between early adverse events and survival while adjusting for other factors that are associated with immunity (e.g., fatigue) and survival (e.g., depression). PATIENTS AND METHODS The patients were recruited from an outpatient oncology clinic. Patients were administered a battery of questionnaires including the Traumatic Events Survey and the Center for Epidemiological Studies-Depression scale. Blood was collected and serum levels of cytokines were assessed to characterize immune status. Descriptive statt for a psychoneuroimmunological model of disease course in this vulnerable population. BACKGROUND AND AIMS The relationship between acute-on-chronic liver failure (ACLF) and acute variceal bleeding (AVB) is poorly understood. Specifically, prevalence and prognosis of ACLF in AVB is unclear, while the role of transjugular intrahepatic porto-systemic shunt (TIPS) in the management in ACLF patients has not been described to date. METHODS A multicenter, international, observational study was conducted including 2138 patients from 34 centers between 2011 and 2015. ACLF was defined and graded according to the EASL-CLIF consortium definition. Placement of pre-emptive TIPS (pTIPS) was based on individual center policy. Patients were followed up to one year, until death or liver transplantation. Cox regression model and competing risk models (Gray’s test) were used to identify independent predictors of rebleeding or mortality. RESULTS At admission, 380/2138 (17.8%) patients had ACLF according to EASL-CLIF criteria (grade-I38.7%; II39.2%; III22.1%). Forty-two day rebleeding rate (19% vs. 10%; p less then 0.001) and mortality (47% vs. 10%; p less then 0.001) were higher in patients with ACLF and increased with ACLF grades. Of note, presence of ACLF was independently associated with rebleeding and mortality. pTIPS placement improved survival in ACLF patients at 42 days and one year. This effect was also observed in propensity score matching analysis of 44 ACLF patients without pTIPS and 22 ACLF patients with pTIPS. CONCLUSIONS This large multi-center international real-life study identified ACLF as at admission an independent predictor of rebleeding and mortality in AVB. Moreover, pTIPS improves survival in patients with ACLF and AVB. Therefore, pTIPS may be considered in ACLF patients with AVB, although the presented data need to be independently validated. OBJECTIVES This study was conducted to examine the tooth wear status of nasopharyngeal-carcinoma (NPC) patients who had received radiotherapy at least 5-year previously, and to investigate the salivary parameters that may be associated with the tooth wear. METHODS Tooth wear status of NPC survivors were clinically assessed using the Exact Tooth Wear Index. A tooth was graded to have severe wear when more than one-third of its buccal/occlusal/lingual surface had dentine loss. At the subject-level, percentages of anterior/posterior/all teeth with severe wear were calculated. Age, number of teeth, flow-rate/buffering capacity/pH of stimulated whole (SWS) and parotid (SPS) saliva’s were collected. Correlation and multiple-linear regression tests were performed at the significance level α = 0.05. RESULT Sixty-eight participants (mean age of 60.0 ± 8.9), 697 anterior and 686 posterior teeth were examined with a mean of 10-years post-radiotherapy. Severe tooth wear was found in 63 (92.6 percent) participants, 288 anterior and 83 posterior teeth.