Entada phaseoloides (Linn.) Merr. (Family Fabaceae) is a well-known, traditional, medicinal plant that has been extensively used in the Ayurvedic system of medicine for centuries to combat a wide range of ailments.

The goal of this work was to investigate the bioactive constituents from n-butanol extracts of Entada. phaseoloides and develop a method for the comprehensive characterization of saponins using liquid chromatography with an electrospray ionization quadrupole time-of-flight mass spectrometer (LC-ESI-QTOF-MS).

A hyphenated technique, ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS/MS), has been proposed to integrate LC and MS together with NMR for structural elucidation. This method allowed comprehensive characterization of saponin glycosides from E. phaseoloides based on their MS/MS fragmentation study.

The phytochemical study of E. phaseoloides resulted in the isolation and identification of three bio-active constituents. Further, the UPLC-Q fragmentation study.Single-cell transcriptomics enables inference of context-dependent phenotypes of individual cells and determination of cellular diversity of complex tissues. Cardiac fibrosis is a leading factor in the development of heart failure and a major cause of morbidity and mortality worldwide with no effective treatment. Single-cell RNA-sequencing (scRNA-seq) offers a promising new platform to identify new cellular and molecular protagonists that may drive cardiac fibrosis and development of heart failure. This review will summarize the application scRNA-seq for understanding cardiac fibrosis and development of heart failure. We will also discuss some key considerations in interpreting scRNA-seq data and some of its limitations.

Bisphenol A (BPA) is a chemical of concern in the food industry. There is a need for a sensitive analytical method for the determination of BPA in beverages.

To develop a method for the determination of BPA in carbonated, non-carbonated, and non-alcoholic drinks.

Replicates of a carbonated soft drink, orange juice with pulp, and a dairy-based coffee drink at spiking levels ranging from 0 to 32 ng/mL were analyzed. The carbonated soft drink was adjusted to pH 7.4 and diluted with phosphate buffered saline (PBS). The orange juice with pulp and the dairy-based coffee drink were extracted with methanol and sodium chloride, then diluted with PBS.

LOD ranged from 0.06 to 0.08 ng/mL and LOQ ranged from 0.10 to 0.14 ng/mL. Recoveries of BPA from all sample types at 1 to 16 ng/mL spiked levels were between 93 and 100%; relative standard deviation (RSDr, %) ranged from 0.71 to 8.38% depending on matrix and spiking levels.

The results indicate that the method for determination of BPA in carbonated, non-carbonated, and non-alcoholic drinks is reproducible and meets AOAC Official MethodSM performance criteria.

The test portions were filtered and the filtrates applied to an immunoaffinity column (IAC) containing antibodies specific for BPA. After the column was washed with water, BPA was eluted from the IAC with 80% methanol and the eluate was directly injected, or concentrated and injected, into ultra-performance liquid chromatography (UPLC) with fluorescence detector (FLD) for separation, detection, and quantitation.

The test portions were filtered and the filtrates applied to an immunoaffinity column (IAC) containing antibodies specific for BPA. After the column was washed with water, BPA was eluted from the IAC with 80% methanol and the eluate was directly injected, or concentrated and injected, into ultra-performance liquid chromatography (UPLC) with fluorescence detector (FLD) for separation, detection, and quantitation.

Current developments in portable photonic devices for fast authentication of extra virgin olive oil (EVOO) or EVOO with non-EVOO additions steer towards hyphenation of different optic technologies. GC376 The multiple spectra or so-called “fingerprints” of samples are then analyzed with multivariate statistics. For EVOO authentication, one-class classification (OCC) to identify “out-of-class” EVOO samples in combination with data-fusion is applicable.

Prospecting the application of a prototype photonic device (“PhasmaFood”) which hyphenates visible, fluorescence, and near-infrared spectroscopy in combination with OCC modelling to classify EVOOs and discriminate them from other edible oils and adulterated EVOOs.

EVOOs were adulterated by mixing in 10-50% (v/v) of refined and virgin olive oils, olive-pomace olive oils, and other common edible oils. Samples were analyzed by the hyphenated sensor. OCC, data-fusion, and decision thresholds were applied and optimized for two different scenarios.

By high-level data-fusion of the classification results from the three spectral databases and several multivariate model vectors, a 100% correct classification of all pure edible oils using OCC in the first scenario was found. Reducing samples being falsely classified as EVOOs in a second scenario, 97% of EVOOs adulterated with non-EVOO olive oils were correctly identified and ones with other edible oils correctly classified at score of 91%.

Photonic sensor hyphenation in combination with high-level data fusion, OCC, and tuned decision thresholds delivers significantly better screening results for EVOO compared to individual sensor results.

Hyphenated photonics and its data handling solutions applied to extra virgin olive oil authenticity testing was found to be promising.

Hyphenated photonics and its data handling solutions applied to extra virgin olive oil authenticity testing was found to be promising.

Determining an athlete’s hydration status allows hydration-related concerns to be identified before significant medical or performance concerns arise. Weight charts are an accurate measure of hydration status changes, yet their clinical use by athletic trainers (ATs) is unknown.

To investigate ATs’ use of weight charts in athletic settings and describe their subsequent clinical decisions.

Cross-sectional survey.

High schools and National Collegiate Athletic Association Divisions I, II, III and National Association Intercollegiate Athletics colleges.

A total of 354 ATs (men = 162, women = 17; 17 respondents did not answer the demographic questions) responded across athletic settings (Division I [45.7%]; Division II, Division III, National Association Intercollegiate Athletics combined [n = 19.9%]; and high school [34.4%]).

The 26-question online survey was developed by content experts and pilot tested before data collection. Participants answered questions focused on weight-chart use (implementation, timing, and calculations) and clinical decision processes (policies, interventions, and referral).