In addition, ultraweak photon emission (UPE) intensity was measured at dazhui point in each subject. RESULTS According to the orthogonal partial least-squares discriminant analysis (OPLS-DA) results and biochemistry databases searching, 15 potential biomarkers were identified to be involved in Spleen-Qi deficiency syndrome and ginseng influenced Spleen-Qi deficiency. The metabonomics and biological experiment data indicated that metabolism pathways were corresponding to energy metabolism, amino acid, carbohydrate, lipid and pyrimidine metabolisms, oxidative stress urea cycle, and intestinal flora metabolism. And the UPE intensity decreased significantly after the treatment. CONCLUSIONS Ginseng could promote the related metabolisms in Spleen-Qi deficiency constitution and the metabolites and UPE intensity may be useful as potential biomarkers for diagnosing and monitoring for Spleen-Qi deficiency syndrome. ETHNOPHARMACOLOGICAL RELEVANCE Many body systems and organs, including the hippocampus, are affected by diabetes, and undergo changes that may increase the risk of cognitive decline. Urtica dioica (UD) has long been recognized as a medicinal plant with beneficial effects on blood glucose control in diabetes. PF-02341066 order AIM OF THE STUDY The present study aimed to investigate the effect of endurance exercise (Ex), along with Urtica dioica (UD) hydro-alcoholic extract on some functional, histological, and molecular aspects of the hippocampus in streptozotocin (STZ)-induced diabetic rats. MATERIALS AND METHODS 60 male Wistar rats were divided into five groups (N = 12) healthy control (H-C), diabetes control (D-C), diabetes exercise (D-Ex), diabetes Urtica dioica (D-UD), and diabetes exercise Urtica dioica (D-Ex-UD). Diabetes was induced intraperitoneally by STZ (45 mg/kg) injection. Two weeks after the injection by STZ, Ex (moderate intensity/5day/week) and gavage of UD extract (50mg/kg/day) was performed for six weeks. Cognitive functions were evaluated by the Morris Water Maze test, routine histological examination, and molecular studies were done via Hematoxylin & Eosin stain, and Western blot. RESULTS Diabetic rats showed spatial learning and memory deficits, as well as negatively affects to the tissue and structure of the hippocampus in the dentate gyrus (DG) and cornu ammonis (CA) areas. Ex + UD treatment caused a decrease of neural disorganization, an increase of neural-microglial density, and thickness of the pyramidal-molecular layer in the hippocampus. In addition, Ex + UD caused a rise of GAP-43 protein levels, a reduction of CAP-1 protein levels, improved hippocampal structure, and improved learning and memory function. CONCLUSIONS These results show that Ex, along with the UD extract, may decrease levels of the central neural complications of diabetes. Given the importance of recognizing non-pharmacological complementary therapies in this field, future studies are warranted. Primary care physicians (PCPs) provide frontline health care to patients in the U.S.; however, it is unclear how their practice styles affect patient care. In this paper, we estimate the long-lasting effects of PCP practice styles on patient health care utilization by focusing on Medicare patients affected by PCP relocations or retirements, which we refer to as “exits.” Observing where patients receive care after these exits, we estimate event studies to compare patients who switch to PCPs with different practice style intensities. We find that PCPs have large effects on a range of aggregate utilization measures, including physician and outpatient spending and the number of diagnosed conditions. Moreover, we find that PCPs have large effects on the quality of care that patients receive, and that all of these effects persist for several years. Our results suggest that switching to higher-quality PCPs could significantly affect patients’ longer-run health outcomes. It is crucial to quantitatively track riverine nitrate (NO3-) sources and transformations in drinking water source watersheds for preventing current and future NO3- pollution, and ensuring a safe drinking water supply. This study identified the significant contributors to riverine NO3- in Zhaoshandu reservoir watershed of Zhejiang province, southeast China. To achieve this goal, we used hydrochemistry parameters and stable isotopes of NO3- (δ15N-NO3- and δ18O-NO3-) accompanied with a Markov Chain Monte Carlo mixing model to estimate the proportional contributions of riverine NO3- inputs from atmospheric deposition (AD), chemical nitrogen fertilizer (NF), soil nitrogen (SN), and manure and sewage (M&S). Results indicated that the main form of riverine nitrogen in this region was NO3-, constituting ~60% of the total nitrogen mass on average (total organic nitrogen ~37% & ammonium ~3%). Variations in the isotopic signatures of NO3- demonstrated that microbial nitrification of NF, SN and M&S was the primary nitro Carlo mixing model to quantify riverine nitrate pollution sources in a drinking water source watershed in Zhejiang province, southeast China. Markov Chain Monte Carlo mixing model output showed that NF, SN and M&S were the dominant sources of riverine NO3- during the sampling period in Zhaoshandu watershed. Uncertainty analysis characterized the variation strength associated with contributions of individual nitrate sources and indicated the greatest uncertainty for SN, followed by NF, M&S and AD. Two temperature-phased anaerobic digestion (TPAD) systems (55 °C in the first reactor and 35 °C in the second reactor) with and without recirculation were operated in parallel for the co-digestion of food waste and paper waste. A long-term experiment was carried out for these two systems with the paper waste ratios elevated from 0 to 50%. The removal efficiencies of COD, TS, VS, carbohydrate and protein in the recirculated TPAD system were higher than those of the non-recirculated system. The successful acclimation of thermophilic cellulose-degrading bacteria in the first reactor (RT1), partly due to recirculation, ensured the effective degradation of cellulose when the paper waste ratio was higher than 40%, resulting in the production of large amounts of hydrogen in reactor RT1. In the absence of recirculation, the main substance produced in the first reactor of the non-recirculated system (T1) was lactic acid. This gradually led to over-acidification and a low degradation efficiency and no methane or hydrogen was produced in T1.