Furthermore, 3-O-β-cellobiosyl-d-glucose showed 237.9% higher antioxidant activity than cellotriose, and laminarin showed 5.06-fold higher antioxidant activity than barley β-glucan, indicating that β-1,4-linkage at reducing end of β-glucans or oligosaccharides resulted in decrease of antioxidant activity compared to β-1,3-linkage.Loxoscelism is the most dangerous araneism form in Brazil and antivenom therapy is the recommended treatment. Antivenom is produced by horse immunization with Loxosceles spider venom, which is toxic for the producer animal. read more Moreover, due to the high amount of venom required for horse hyperimmunization, new strategies for antigens obtention have been proposed. In this sense, our research group has previously produced a non-toxic recombinant multiepitopic protein derived from Loxosceles toxins (rMEPLox). rMEPLox was a successful immunogen, being able to induce the production of neutralizing antibodies, which could be used in the Loxoscelism treatment. However, rMEPLox obtention procedure requires optimization, as its production needs to be scaled up to suit antivenom manufacture. Therefore, an effective protocol development for rMEPlox production would be advantageous. To achieve this objective, we evaluated the influence of different cultivation conditions for rMEPLox optimum expression. The optimum conditions to obtain large amounts of rMEPlox were defined as the use of C43(DE3)pLysS as a host strain, 2xTY medium, 0.6 mM IPTG, biomass pre induction of OD600nm = 0.4 and incubation at 30 °C for 16 h. Following the optimized protocol, 39.84 mg/L of soluble rMEPLox was obtained and tested as immunogen. The results show that the obtained rMEPLox preserved the previously described immunogenicity, and it was able to generate antibodies that recognize different epitopes of the main Loxosceles venom toxins, which makes it a promising candidate for the antivenom production for loxoscelism treatment.Fucan sulfates from echinoderm possess characteristic structures and various biological activities. Herein, comprehensive methods including enzymolysis, ion-exchange chromatography and size exclusion chromatography lead to the purification of five fucan sulfates (FSI, FSII, FSIII, FSIV, FSV) from the sea cucumber Pattalus mollis. Chemical composition analysis showed that they were all composed of l-fucose. Their sulfate content was determined by a conductimetric method. The molecular weight (Mw) of FSI, FSII, FSIII, FSIV and FSV were measured as 238.3 kDa, 81.0 kDa, 82.0 kDa, 23.2 kDa and 6.12 kDa, respectively. Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → . This is the first report that such a diversity of fucan sulfates were obtained from the same sea cucumber species. Biological activity showed that FSI, FSII, FSIII and FSIV exhibited potent anticoagulant by prolonging the APTT. Among them, FSII, FSIII and FSIV showed the similar potency, while FSI owned the strongest. Structure-activity relationships analysis showed that molecular weight and sulfation degree should be the crucial factors for the activity.The efficient use of waste from food processing industry is one of the innovative approaches within sustainable development, because it can be transferred into added value products, which could improve economic, energetic and environmental sectors. In this context, the squid pen waste from seafood industry was used as raw material to obtain nanofibrous β-chitin films. In order to extend functionality of obtained films, elderberry extract obtained from biomass was added at different concentrations. The tensile strength of chitin-elderberry extract films was improved by 52%, elongation at break by 153% and water vapor barrier by 65%. The obtained material showed distinct color change when subjected to acidic or basic solutions. It was proven by CIELab color analysis that all color changes could be easily perceived visually. In addition, the obtained nanofibrous film was successfully used to monitor the freshness of Hake fish. Namely, when the film was introduced in a package that contained fresh fish, its color was efficiently changed within the time during the storage at 4 °C. The obtained results demonstrated that food processing waste could be efficiently valorized, and could give sustainable food package design as a spoilage indicator of high protein food.The development of composite films with enhanced antibacterial and dye decolorization properties for water treatment has attracted a great attention. In this study, nickel oxide/chitosan/polyvinyl alcohol nanocomposite films containing different weight percentage of NiO nanoparticles with a dual functionality, removal of toxic dye and antibacterial properties, were prepared. Methyl orange (MO) was selected as a target pollutant. Additionally, the antimicrobial activity of the films against two Gram positive bacteria (Staphylococcus aureus and Bacillus cereus) and two Gram negative bacteria (Escherichia coli and Salmonella Typhimurium) was studied. The prepared samples were characterized by XRD, HRTEM, FESEM, ATR-FTIR, UV-Vis spectroscopy, and dielectric measurements. The morphological examination proved that the nanocomposite film has more porous structure compared to the unmodified chitosan/PVA. The antimicrobial tests indicated that the modified chitosan/PVA films have higher activity than pure chitosan/PVA toward all the tested pathogenic bacteria. The impact of the NiO amount (0.5, 1.5, 3, and 5 wt%), contact time (0-150 min), and adsorbent dose (40, 80, and 100 mg) on the removal of MO was studied. Dye adsorption results proved that the incorporation of 5 wt% NiO led to more than 2 fold increase in the dye removal percentage in comparison with the unmodified PVA/chitosan film.