P. stipitis (NCIM3498) yielded maximum ethanol of 24 g/L with ethanol yield of 0.455 ± 0.02 g/g substrate and a productivity of 1.004 ± 0.050 g/L/h after 24 h of fermentation. With concentrated acid hydrolysate as substrate, S. cerevisiae (VS3) produced ethanol of 8.52 ± 0.43 g/L, whereas S. cerevisiae (NCIM3455) produced 5.96 ± 0.30 g/L of ethanol. P.stipitis (NCIM3498) produced 4.52 ± 0.23 g/L of ethanol by utilizing 14.66 ± 0.87 g/L of sugars. Co-culture with S. cerevisiae (VS3) addition after 18 h of addition of P. stipitis (NCIM3498) to the mixture of concentrated acid hydrolysate and enzyme hydrolysate produced 13.86 ± 0.47 g/L of ethanol with fermentation efficiency, ethanol yield and productivity of 87.54 ± 0.54%, 0.446 ± 2.36 g/g substrate and 0.385 ± 0.014 g/L/h, respectively. Hence, it is concluded that co-culture with S. cerevisiae and P. stipitis is feasible, further scaling up of fermentation of P. juliflora substrate for bioethanol production.Colle totrichum falcatum, an intriguing pathogen causing red rot in sugarcane, exhibits enormous variation for pathogenicity under field conditions. A species-specific marker is very much needed to classify the virulence among the varying population and to identify the potential of a pathotype by mining the microsatellites, which are considered to be the largest genetic source to develop molecular markers for an organism. In this study, we have mined the C. falcatum genome using MISA database which yielded 12,121 SSRs from 48.1 Mb and 2745 SSRs containing sequences. The most frequent SSR types from the genome of C. falcatum was di-nucleotide which constitutes 50.89% followed by tri-nucleotide 39.60%, hepta-nucleotide 6.7%, hexa-nucleotide 1.38% and penta-nucleotide 1.3%. Over 90 SSR containing sequences from the genome were predicted using BlastX which are found to be non-homologs. Most of the annotated SSR containing sequences fell in CAZy superfamilies, proteases, peptidases, plant cell wall degrading enzymes (PCDWE) and membrane transporters which are considered to be pathogenicity gene clusters. Among them, glycosyl hydrolases (GH) were found to be abundant in SSR containing sequences which again proved our previous transcriptome results. Our in-silico results suggested that the mined microsatellites from C. falcatum genome show absence of homolog sequences which suggests that these markers could be used as an ideal species-specific molecular marker. Two virulence specific markers were characterized using conventional PCR assays from C. falcatum along with virulent species-specific (VSS) marker developed for C. gloeosporioides. The study lays the foundation for the development of C. falcatum specific molecular marker to phenotype the pathotypes based on virulence.Sucrose non-fermenting 1 (SNF1) is a protein kinase and plays an important role in the energy homeostasis of glucose repressible gene transcription. It derepresses glucose repressed genes and associated with pathogenesis and production of cell wall degrading enzymes in fungal species. In the present study, we identified and characterized SNF1 homologue FuSNF1 in the F. udum strain WSP-V2. I-BET151 Transcript analysis of FuSNF1 along with the MAP kinases and some cell wall degrading enzyme (CWDE) genes of F. udum during interaction with pigeonpea revealed that most MAP kinases and CWDE genes was positively correlated with the FuSNF1 gene. Interestingly, transcript accumulation of all these genes was lowered when pigeonpea seeds were bioprimed with a PGPR strain Pseudomonas fluorescens OKC. Transcript accumulation of FuSNF1 was observed from the day of inoculation and reached maximum level on day 7 in OKC non-bioprimed plants. However, transcript accumulation was low (1.5 fold) in F. udum inoculated with pigeonpea plants bioprimed with OKC. Transcript accumulation patterns of the F. udum MAP Kinases genes and CWDE genes also showed a similar trend and their transcript accumulation was lowered in the OKC bioprimed treatment. The results thus indicate a prime role of FuSNF1 in regulating pathogenicity and virulence of F. udum. The results further emphasize the importance of application of effective PGPR strains in regulating virulence of F. udum. In silico analysis of the SNF1 reference proteins from different fungal species showed that their homologue FuSNF1 is likely to be thermostable and acidic in nature.The aim was to investigate the effect of lily bulbs on the microecological characteristics of intestinal microbiota and enzyme activities in normal mice. Thirty SPF Kunming mice were randomly divided into the control group, Lilium lancifolium (LL) group and Lilium davidii var. unicolor (LDU) group. Mice of the latter two groups were given 0.15 g·mL-1 lily bulb solution, respectively, by gavage twice a day, while the control group was given the same volume of sterilized water. After 49 days, intestinal contents and mucosa of all mice were collected and the characteristics of intestinal microbiota and enzyme activities were analyzed. Results showed that the number of Lactobacillus spp. and Bifidobacteria spp. in the LL group was significantly higher than that in the control group (t = 2.68 × 107, P = 0.000; t = 5.96 × 107; P = 0.000) and the LDU group (t = 6.12 × 107, P = 0.000; t = 2.71 × 107, P = 0.000), while the number of total bacteria was significantly lower (P = 0.040). Microbial activity in intestinal cteria spp., and inhibit the growth of total bacteria in the intestines of normal mice. Lilium lancifolium bulbs have the potential to be a functional food.This study was aimed at the genome-wide identification, a comprehensive in silico characterization of NHX genes from soybean (Glycine max L.) and their tissue-specific expression under varied levels (0-200 mM NaCl) of salinity stress. A total of nine putative NHX genes were identified from soybean. The phylogenetic analysis confirmed a total of five sub-groups and GmNHXs were distributed in three of them. Bioinformatics analyses confirmed all GmNHXs as ion transporters in nature, and all were localized on the vacuolar membrane. Several cis-acting regulatory elements involved in hormonal signal-responsiveness and abiotic stress including salinity responses were identified in the promoter regions of GmNHXs. Amiloride, which is a known Na+/H+ exchanger activity inhibitor, binding motifs were observed in all the GmNHXs. Furthermore, the identified GmNHXs were predicted-targets of 75 different miRNA candidates. To gain an insight into the functional divergence of GmNHX transporters, qRT-PCR based gene expression analysis was done in control and salt-treated root, stem and leaf tissues of two contrasting Indian soybean varieties MAUS-47 (tolerant) and Gujosoya-2 (sensitive).