During 4- and 8-week observation periods, the PKH 26 appeared green (fluorescent).

 hUCMSC demonstrates high-osteogenic activity and increased osteoporotic mandibular bone regeneration, as shown by increased expression of Osx and BMP-2 and decreased TRAP expression. From the labeling, PKH-26 proved that viable hUCMSCs in gelatin solvent can be present in the mandibular bone and be capable of promoting osteogenic differentiation and increasing mineralization and bone formation in the osteoporotic mandibular bone.

 hUCMSC demonstrates high-osteogenic activity and increased osteoporotic mandibular bone regeneration, as shown by increased expression of Osx and BMP-2 and decreased TRAP expression. From the labeling, PKH-26 proved that viable hUCMSCs in gelatin solvent can be present in the mandibular bone and be capable of promoting osteogenic differentiation and increasing mineralization and bone formation in the osteoporotic mandibular bone.

 This study aimed to analyze the effectiveness of three irrigation systems EndoActivator, passive ultrasonic irrigation (PUI), and Easy Clean in the reduction of

, after instrumentation with the reciprocating system, through microbiological collection and culture method.

 A total of 60 extracted human lower premolars were used and standardized at 16 mm in length. The teeth were accessed, contaminated with

, and incubated for 21 days at 37°C. Initial collections (S1) were made with an absorbent paper cone to confirm the contamination; subsequently, instrumentation was performed with WaveOne Primary. The teeth were divided into four groups according to the final irrigation protocol (

= 15) group 1. EndoActivator; group 2, PUI; group 3, Easy Clean; and group 4, control group irrigated with saline solution sterile and without agitation. In the final irrigation, the agitation of the 17% ethylene amine tetra-acetic acid (EDTA) solutions was used, then 2.5% sodium hypochlorite (NaOCl); in both for this, three cycles of 20 seconds each. After the chemical-mechanical preparation and agitation of the irrigating solutions, the final collections (S2) for counting the colony-forming units (CFU/mL) occurred.

 The Kruskal-Wallis test revealed that all the agitation systems reduced by 100% and the control group by 65.7%.

 The control group presented a significantly higher amount of CFU/mL after the chemical-mechanical preparation than the other groups, which were similar to each other (

> 0.05).

0.05).

 Planning, adequate preparation, and further prosthodontic management are the most important steps in the treatment of this population of patients with dental and mandibular anomalies. The purpose of the article is the improvement of safety and clinical effectiveness of prosthodontic rehabilitation of patients after full-mouth reconstruction.

 A single-center open prospective nonrandomized study, examination, and treatment of 198 patients with partial or total absence of teeth were performed. Higher safety and clinical effectiveness of the treatment were found in the patients of the main group who showed a significant reduction in the rate of complications relative to the comparison group. click here In the main group, the number of aesthetic defects and cases of repeated prosthetics decreased in comparison with the corresponding occurrence rate of such cases in patient groups.

 It showed statistically significantly reduction in the rates of occurrence of the complications when using the proposed algorithm the ratneed for repeated prosthetic treatment was reduced in comparison with the corresponding frequency of such cases in groups of patients where standard approaches to prosthetics were applied. In particular, aesthetic defects found in the main group of patients were 2.5 times less frequent than in the comparison group, and repeated prosthetics was performed 2.7 times less frequently.

 Application of the developed comprehensive approach to planning of prosthodontic rehabilitation provides for significant reduction in the frequency of complications and aesthetic defects, as well as cases of repeated need for prosthetics.

 Application of the developed comprehensive approach to planning of prosthodontic rehabilitation provides for significant reduction in the frequency of complications and aesthetic defects, as well as cases of repeated need for prosthetics.

Conduct a histological comparison of the pulp response to different materials, with a focus on the continuity and morphology of the mineralized barrier after direct pulp capping.

 One hundred and eight maxillary first molars of 54 Wistar rats were subject to direct pulp capping and divided into three groups according to the materials used calcium hydroxide (CH), mineral trioxide aggregate (MTA), and Biodentine. All cavities were sealed, and the animals were euthanized at 7, 14, and 21 days. Descriptive histological evaluation of the inflammation and formation of the mineralized barrier was performed.

 Statistical analyses were performed using the Kruskal-Wallis test, which was complemented by the Dunn test; differences with

< 0.05 were considered statistically significant.

 The results showed that MTA and Biodentine elicited less intense inflammatory reactions than CH. With respect to the formation and quality of the dentin barrier formed, differences were observed at 21 days between the analyzed groups; the best results being obtained following treatment with MTA and Biodentine.

 MTA and Biodentine induced formation of a more continuous and uniform mineralized barrier with less intense pulp response than CH.

 MTA and Biodentine induced formation of a more continuous and uniform mineralized barrier with less intense pulp response than CH.

 This article aimed to study the effect of different concentrations of nano zinc oxide particles on the color change of MDX4-4210 facial silicone elastomer after artificial aging.

 Silicone specimens (

= 150) were fabricated by incorporating intrinsic pigments and divided into three groups-white, yellow, and red, each group consisting of 50 specimens (

= 50). In each color, specimens were subdivided into five subgroups according to the quantity of zinc oxide nanoparticles (0, 0.5, 1.0, 1.5, and 2.0% weight), where the 0% weight served as the control in each group. All specimens were then subjected to artificial aging using an accelerated aging machine chamber for 12, 24, 48, and 72 hours. L*a*b* values of specimens were noted after a different aging period by a spectrophotometer and ∆

* was calculated.

 Two-way repeated analysis of variance (ANOVA) was done to examine the effects under test conditions (concentration and aging time) of each color group. Then color, concentration, and the aging period were subjected to three-way repeated ANOVA to investigate the effects of different colors and concentrations on ∆

*.