Intra-day and inter-day precisions (relative standard deviation) were <10%. Ang II, Ang III, Ang IV, and Ang (1-5) were positively correlated with ACE expression by HUVECs, while Ang I, Ang (1-7), and Ang (1-9) were negatively correlated.

The nanoflow liquid chromatography-parallel reaction monitoring mass spectrometry-based methodology established in this study can evaluate the Ang peptides simultaneously in HUVEC culture supernatant.

The nanoflow liquid chromatography-parallel reaction monitoring mass spectrometry-based methodology established in this study can evaluate the Ang peptides simultaneously in HUVEC culture supernatant.

Implementing sustainable farming practices for winter wheat (

L.) in the North China Plain may be a way to reduce carbon emissions. No tillage generally results in less net CO

loss from farmland, but no tillage also reduces the grain yield and water use efficiency (WUE) of winter wheat. Wide-precision planting of winter wheat may enhance the grain yield and WUE; however, it is not known precisely how tillage and planting patterns affect CO

exchange, grain yield and WUE.

In this study, two tillage methods (conventional tillage, T and no tillage, NT) and two planting patterns (conventional planting, C and wide-precision planting, W) were used in two consecutive winter wheat growing seasons.

Compared with the T treatments, the NT treatments had significantly lower cumulative net CO

emissions in 2015-2016 and 2016-2017 (30.8 and 21.3%, respectively), and had lower grain yields (9.0 and 9.4%, respectively) and WUE (6.0 and 7.2%, respectively). The W treatments had a compensating effect on grain yieldefficiency (YCUE). The lowest cumulative CO2 emissions and highest YCUE were observed for NT with W treatment. Results from this analogous tillage experiment indicated that NT and W farming practices provide an option for reducing carbon emissions and enhancing WUE and YCUE for sustainable winter wheat development.

Growing evidence indicates that proanthocyanidins (PACs) may be effective in treating and preventing various cancers. The fundamental mechanism of PACs inhibiting the proliferation at cellular and molecular levels in most of the cancer types remains unclear.

The anticancer efficacy of PACs was investigated in vitro using three human cancer cell lines human colorectal adenocarcinoma (HT-29), human breast carcinoma (MCF-7), and human prostatic adenocarcinoma (PC-3).

Cytotoxicity was evaluated by MTT assay, while cell proliferation was measured by trypan blue exclusion method. Cell migration was measured by wound healing assay, and DAPI staining was used to evaluate apoptotic nucleus morphology. RT-PCR was used to analyze the expression of

and

, and caspase enzyme activity assay was measured by caspase colorimetric assay.

PACs could inhibit both cellular viability and proliferation in a concentration- and time-dependent fashion in all investigated cells. Further, all tested cells showed similarly decreased migration after 24- and 48-h PAC treatment. We observed increased apoptotic nucleus morphology in treated cells (

≤0.01).

expression significantly increased in HT-29 (

<0.01), PC-3(

<0.01), and MCF-7 (

<0.05) cells, while

expression significantly declined (

<0.05). Caspase activities were significantly increased in all tested cancer cell lines after 24-h PAC treatment.

PACs may have potential therapeutic properties against colorectal, breast, and prostate cancer.

PACs may have potential therapeutic properties against colorectal, breast, and prostate cancer.Fruit cracking is a physiological disorder in many plant species that leads to severe economic losses. this website The aim of this study was to investigate the effect of calcium on fruit cracking and explore the underlying mechanisms. We studied the effect of exogenous calcium on grape berry cracking, calcium absorbance and distribution, and cell wall metabolism in the cracking-susceptible cultivar ‘Xiangfei’. Calcium significantly reduced the frequency of fruit cracking, increased the break force of the berry skin, and stimulated storage of calcium. In addition, calcium increased the content of protopectin and inhibited the increase in content of water-soluble pectin, by regulating the transcription and activities of enzymes associated with cell wall metabolism. Taken together, the results indicated that dipping grape berries in calcium solution is effective in preventing fruit cracking by stimulating calcium uptake, inhibiting cell wall disassembly, and promoting cell wall strengthening.N6-methyladenosine (m6A) is the most prevalent modification in eukaryotic mRNA and potential regulatory functions of m6A have been shown by mapping the RNA m6A modification landscape. m6A modification in active gene regulation manifests itself as altered methylation profiles. The number of reports regarding to the profiling of m6A modification and its potential role in the placenta of preeclampsia (PE) is small. In this work, placental samples were collected from PE and control patients. Expression of m6A-related genes was investigated using quantitative real-time PCR. MeRIP-seq and RNA-seq were performed to detect m6A methylation and mRNA expression profiles. Gene ontology (GO) functional and Kyoto encyclopedia of genes and genomes (KEGG) pathway analyses were also conducted to explore the modified genes and their clinical significance. Our findings show that METTL3 and METTL14 were up-regulated in PE. In total, 685 m6A peaks were differentially expressed as determined by MeRIP-seq. Altered peaks of m6A-modified transcripts were primarily associated with nitrogen compound metabolic process, positive regulation of vascular-associated smooth muscle cell migration, and endoplasmic reticulum organisation. The m6A hyper-methylated genes of Wnt/β-catenin signalling pathway, mTOR signalling pathway, and several cancer-related pathways may contribute to PE. We also verified that the significant increase of HSPA1A mRNA and protein expression was regulated by m6A modification, suggesting m6A plays a key role in the regulation of gene expression. Our data provide novel information regarding m6A modification alterations in PE and help our understanding of the pathogenesis of PE.