BACKGROUND AND AIMS Mixed hepatocellular-cholangiocarcinoma (HCC-CC) is a biphenotypic liver cancer thought to have unfavorable tumor biology and a poor prognosis. Surgical outcomes of HCC-CC remain unclear. We aimed to evaluate the clinical and characteristics and surgical outcomes of HCC-CC. APPROACH AND RESULTS Case series of patients undergoing resection for HCC-CC (n=47), hepatocellular carcinoma (HCC) (n=468), and intrahepatic cholangiocarcinoma (ICC) (N=108) at a single Western center between 2001 and 2015. Cases of HCC-CC were matched to cases of HCC and ICC on important clinical factors including tumor characteristics (size, vascular invasion, differentiation), and underlying cirrhosis. Patients with HCC-CC had rates of viral hepatitis comparable to patients with HCC (78.5% vs. 80%) and 42.5% had underlying cirrhosis. When matched on tumor size, HCC-CC was more poorly differentiated than HCC (68.3% vs. 27.3%, respectively; P less then 0.001) and ICC (68.3% vs. 34.8%, respectively, P=0.01) but had similar post-resection survival (5-year survival HCC-CC 49.7%, HCC 54.8%, ICC 68.7%, P=0.61) and recurrence (3-year recurrence HCC-CC 57.9%, HCC 61.5%, and ICC 56%, P=0.55). Outcomes were similar between HCC-CC and HCC when matched on underlying cirrhosis and tumor size. Cancer type was not predictive of survival or tumor recurrence. CONCLUSIONS Survival after resection of HCC-CC is similar to HCC when matched for tumor size, despite HCC-CC tumors being more poorly differentiated. Exclusion of HCC-CC from management strategies recommended for HCC, including consideration for liver transplantation, may not be warranted. This article is protected by copyright. All rights reserved.Although mice are widely used to elucidate factors contributing to penile disorders and develop treatment options, quantification of tissue changes upon intervention is either limited to minuscule tissue volume (histology) or acquired with limited spatial resolution (MRI/CT). Thus, imaging method suitable for expeditious acquisition of the entire mouse penis with subcellular resolution is described that relies on both aqueous- (CUBIC) and solvent-based (FDISCO) tissue optical clearing (TOC). The combined TOC approach allows to image mouse penis innervation and vasculature with unprecedented detail and, for the first time, reveals the three-dimensional structure of murine penis fibrocartilage. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.OBJECTIVE Adoption of emergency department (ED)-initiation of buprenorphine (BUP) for opioid use disorder (OUD) into routine emergency care has been slow, partly due to clinicians’ unfamiliarity with this practice and perceptions that it is complicated and time-consuming. To address these barriers and guide emergency clinicians through the process of BUP initiation, we implemented a user-centered computerized clinical decision support system (CDS). This study was conducted to assess the feasibility of implementation and to evaluate the preliminary efficacy of the intervention to increase the rate of ED-initiated BUP. METHODS An interrupted time series study was conducted in an urban, academic ED from April 2018-February 2019 (pre-implementation phase), March 2019-August 2019 (implementation phase), and September 2019-December 2019 (maintenance phase) to study the effect of the intervention on adult ED patients identified by a validated EHR-based computable phenotype consisting of structured data consistent wiates, the relative risk of BUP initiation rate was 2.73 (95% CI 0.62, 12.0; p=0.18). Similarly, the number of unique attendings who initiated BUP increased modestly 7/53 (13.0%) to 13/57 (22.8%, p=0.10) after offering just-in-time training during the implementation period. The rate of naloxone prescribed at discharge also increased (6.5%, pre-implementation; 11.5%, implementation; p less then 0.01). The intervention received a System Usability Scale score of 82.0 (95% CI 76.7-87.2). CONCLUSION Implementation of user-centered CDS at a single ED was feasible, acceptable, and associated with increased rates of ED-initiated BUP and naloxone prescribing in patients with OUD and a doubling of the number of unique physicians adopting the practice. We have implemented this intervention across several health systems in an ongoing trial to assess its effectiveness, scalability, and generalizability. This article is protected by copyright. All rights reserved.A copper-catalyzed electrophilic etherification of arylboronic esters is reported. EGFR assay Isoxazolidines are utilized as easily available and stable [RO] + surrogates to give 1,3-amino aryl ethers. The O -selective arylation of isoxazolidines takes place without causing competitive N -arylation. In contrast to previously reported anionic conditions, our copper-catalyzed conditions are mild enough to achieve high functional group tolerance. Preliminary mechanistic studies and DFT calculations support that the reaction proceeds via a transmetalation/oxidative addition pathway, followed by a Lewis acid-promoted reductive elimination to induce the crucial O -selectivity. © 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.BACKGROUND Hospital affiliated freestanding emergency departments (FREDs) are rapidly proliferating in some states and have been the subject of recent policy debate. As FREDs’ role in acute care delivery is expanding in certain regions, little is known about the quality of care that they provide for their sickest patients. Our aim was to compare timeliness of emergent care at FREDs and hospital-based EDs (HEDs) for patient visits with selected high acuity and time-sensitive conditions. METHODS We performed a retrospective observational analysis of adult patient visit data from 19 FREDs and five HEDs from one health system over a one-year period. Median time-to-events and hazard ratios (HRs) with 95% confidence intervals (CIs) were calculated via cox regression. RESULTS The median time to electrocardiogram for visits with chest pain was 10 minutes at FREDs and 9 minutes at HEDs [HR 0.91 (CI 0.87 – 0.96)]. Time to cardiac catheterization lab for visits with ST-segment elevation myocardial infarction (STEMI) was 78 minutes at FREDs, inclusive of transfer time, and 31 minutes at HEDs [HR 0.