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  • Axelsen Drew posted an update 3 days, 7 hours ago

    Altogether, the phylogenetic data and the chemotaxonomic characteristics of this strain support that aSej3T represents a new species for which we propose the name Bradyrhizobium hipponense sp. nov. with the type strain aSej3T (=DSM 108913T=LMG 31020T).A Gram-stain-negative, aerobic, non-motile and ovoid- to rod-shaped bacterial strain, designated GH877T, was isolated from a water sample of Gahai saline lake in Qaidam Basin,PR China. The isolate grew at 5-45 °C, pH 6.0-9.0 (optima, 37 °C and pH 7.5) and with 0.5-20 % (w/v) NaCl (optimum, 2.0 %). The neighbour-joining phylogenetic tree of 16S rRNA gene sequences showed that strain GH877T belonged to the genus Roseovarius, and had highest 16S rRNA gene sequence similarity of 97.7 % to Roseovarius pacificus 81-2T, followed by Roseovarius halotolerans HJ50T (97.5 %) and Roseovarius litoreus GSW-M15T (96.8 %). Genome sequencing revealed a genome size of 3 378 519 bp and a G+C content of 59.8 mol %. Up-to-date bacterial core gene set analysis indicated that strain GH877T represents one independent lineage with R. pacificus DSM29589T. The average nucleotide identity values of GH877T with R. pacificus 81-2T and R. halotolerans HJ50T are 80.7 and 77.3 %, respectively. In silico DNA-DNA hybridization values between strain GH877T and R. pacificus 81-2T and R. halotolerans HJ50T are 23.2 and 20.0 %, respectively. Q-10 was the predominant respiratory quinone and summed feature 8 (C18    1 ω7c and/or C18    1 ω6c) and C16    0 were the major cellular fatty acids. The polar lipids of strain GH877T consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and two unidentified phosphoglycolipids. Based on polyphasic taxonomic analysis, strain GH877T is proposed to represent a novel species of the genus Roseovarius, for which the name Roseovarius gahaiensis sp. nov. is proposed. (type strain GH877T=CGMCC 1.13971T=KCTC 72576T).Purpose The purpose of this study was to examine the relationships linking motivational variables with BMI percentile (BMI%) via segmented physical activity (PA) and sedentary behavior (SB) in adolescents using path analysis. Method Secondary data analysis was performed using participants from the Family, Life, Activity, Sun, Health, and Eating (FLASHE) study. A non-probability panel balanced to the US population on sex, Census division, household income and size, and race/ethnicity were screened for eligibility. Isoxazole 9 concentration The final sample consisted of 1,643 adolescents (822 girls, 821 boys). Motivational variables consisted of PA enjoyment, self-efficacy, and peer social support. Mediator variables consisted of segmented weekly PA and SB assessed using the Youth Activity Profile (YAP). BMI% was the outcome. A recursive bootstrapped path analysis was conducted to examine the relationships between motivational variables, segmented PA and SB, and BMI% with indirect effects calculated via bootstrapped mediation analyses. Results The relationships between motivational variables and segmented PA and SB were stronger than relationships between segmented PA and SB and BMI%. Three mediated paths were observed self-efficacy and BMI% using sedentary YAP as the mediator (IE = -0.38, 95%CI -0.65, -0.18), enjoyment and BMI% using weekend YAP as the mediator (IE = -0.62, 95%CI -1.14, -0.12) and social support and BMI% using weekend YAP as the mediator (IE = -0.53, 95%CI -1.00, -0.07). Conclusion The relationship between adolescent motivational variables and segmented PA and SB is relatively strong and specific motivational variables’ relationships with BMI% is mediated through segmented PA and SB.To explore pain complaints and health-related conditions, verifying if permanent or temporary usage of forearm crutches could be associated with them. We designed a cross-sectional study from a sample who answered a five-month public call. We organized data into five domains (1) diseases, signs and symptoms; (2) personal factors related to age, sex, marital status, and paid occupation; (3) body structure and functional components defined by body mass index, arterial pressure, mental state, and pain; (4) activities and participation assessed by satisfaction with Assistive Technology; (5) and environmental factors focused on medicines and forearm crutch usage. The sample was geo-referenced by address, and the frequency of the codified health conditions was distributed according to ICD-10’s chapters. We recruited three times more permanent than temporary users dealing with chronic and external causes of diseases. Pain mapping suggested different pattern of complaints between permanent and temporary users. Women who were temporary users seemed more likely to be injured because of external causes. Moreover, both users reported intense (31%) and moderate (53%) levels of pain. In contrast, mild pains were only reported by permanent users (16%), suggesting a distinction between acute and chronic pain according to the kind of forearm crutch usage.Evaluating alternate hosts that facilitate the persistence of a virus in the landscape is key to understanding virus epidemics. In this study, we explored the role of several plant species (eggplant, pepper, and Palmer amaranth) as inoculum sources of tomato yellow leaf curl virus (TYLCV) and as reservoirs for its insect vector, Bemisia tabaci (Gennadius). All inoculated species were infected with TYLCV, but whiteflies acquired fewer viral copies via feeding from pepper and eggplant than from tomato and Palmer amaranth. Further, back-transmission assays to recipient tomato resulted in TYLCV infection only when TYLCV was acquired from Palmer amaranth or tomato. Analysis suggested that the role of plant species as TYLCV inoculum sources may be determined by the accumulation of viral copies in the plant, and consequently in the insect vector. In addition, results showed that all three alternate species could sustain populations of B. tabaci, while differentially influencing fitness of whiteflies. Eggplant was a superior host for whiteflies, whereas whitefly survival was compromised on pepper. Together, we demonstrate that both plant-virus and plant-vector interactions could influence the role of an alternate host in TYLCV epidemics, and in our region of study we highlight the potential risk of hosts such as Palmer amaranth in the spread of TYLCV.

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