Yinjiao (GV 28) is generally used in treatment of lumbago and anorectal diseases. Professor LIN Guo-hua believes that Yinjiao (GV 28) is indicated in the disorders related to the sea of marrow and lower jiao by regulating the governor vessel and the conception vessel, promoting qi and blood circulation, tonifying the sea of marrow and adjusting the lower jiao. Based on professor Lin’s clinical experience, “three Yinjiao” points are innovated, named Yinjiao (GV 28, upper Yinjiao), external Yinjiao and lower Yinjiao. Acupuncture therapy is generally optioned at Yinjiao (GV 28) and 5 needling principles are emphasized, i.e. accurate acupoint location, strong needling sensation, needling combined with body movement, alternative stimulation and allied stimulation. All of those mentioned above provide a new approach to the clinical treatment for the disorders related to the sea of marrow and lower jiao.

To observe the effect of acupuncture technique of

on learning-memory ability and expressions of hippocampal vascular endothelial growth factor (VEGF) and angiogenin-1 (Ang-1) in rats with vascular dementia (VD), and to explore the mechanism of acupuncture technique of

for VD.

A total of 24 male SD rats were randomly divided into a sham operation group, a model group, a medication group and an acupuncture group after Morris water maze test, 6 rats in each group. VD model was established by permanent ligation of bilateral common carotid arteries in the model group, the medication group and the acupuncture group. Treatment was given on the next day after successful modeling. The rats in the acupuncture group were treated with acupuncture at “Baihui” (GV 20), “Shenting” (GV 24), “Shuigou” (GV 26), “Dazhui” (GV 14), “Fengfu” (GV 16), “Mingmen” (GV 4), “Neiguan” (PC 6), “Daling” (PC 7) and “Laogong” (PC 8) for 30 min; the rats in the medication group were treated with nimodipine solution (0.0625 g/kg) by increased (

<0.01,

<0.05).

The acupuncture technique of

can significantly improve the learning and memory ability of VD rats, and its mechanism may be related to up-regulating the expressions of VEGF and Ang-1 protein in hippocampus and inducing angiogenesis.

The acupuncture technique of Tiaoxin Tongdu can significantly improve the learning and memory ability of VD rats, and its mechanism may be related to up-regulating the expressions of VEGF and Ang-1 protein in hippocampus and inducing angiogenesis.

To observe the effect of electroacupuncture (EA) preconditioning on the expressions of nuclear transcription factors-kappa B (NF-κB) p65, NF-κB inhibitor (IκB) α and IκB kinase (IKK) β in rats with acute myocardial ischemia-reperfusion injury (MIRI) and to explore the mechanism of EA on heart meridian in relieving MIRI.

A total of 40 SD rats were randomized into a sham-operation group, a model group, an EA heart meridian group and an EA lung meridian group, 10 rats in each one. In the EA heart meridian group, acupuncture was applied to “Shenmen” (HT 7) and “Tongli” (HT 5). In the EA lung meridian group, acupuncture was applied to “Taiyuan” (LU 9) and “Lieque” (LU 7). In these two groups, EA was exerted for 20 min each time, 1 V in voltage and 2 Hz in frequency once a day. A total of 7-day EA stimulation was required before model duplication. In the model group, the EA heart meridian group and the EA lung meridian group, using ligating left anterior descending coronary artery to establish the acute MIRI mo signaling pathway possibly participates in the protective mechanism of electroacupuncture preconditioning on acute MIRI.

Electroacupuncture preconditioning at heart meridian acupoints obviously alleviates acute MIRI. IKK/IκB/NF-κB signaling pathway possibly participates in the protective mechanism of electroacupuncture preconditioning on acute MIRI.

To investigate the effect of electroacupuncture (EA) at different acupoint combination on intestinal motility and expression of inflammatory factors IL-6 and TNF-α in intestinal muscle layer in mice with postoperative ileus (POI), so as to provide reference of acupoint combination of clinical EA intervention for POI.

A total of 78 SPF-grade male C57BL/6 mice aged 8-12 weeks were randomly divided into a sham operation group, a model group, a ST 36+ non-acupoint group, a ST 36+ ST 25 group, a ST 36+ CV 4 group and a ST 36+ CV 12 group, 13 mice in each group. SANT-1 The model of POI was established by intestinal manipulation. The mice in each acupuncture group were intervened with HANS-200A acupoint nerve stimulator within 3 hours after modeling, continuous wave, frequency of 10 Hz, intensity of 1 mA, for 20 min. The EA was given once. 22.5 h after model establishment, 7 mice in each group were intragastrically administered with fluorescein isothiocyanate-conjugated (FITC-dextran). 1.5 h after intragastric administps (

>0.05).

EA at “Zusanli” (ST 36) or “Zusanli” (ST 36) combined with “Tianshu” (ST 25) can improve intestinal motility in mice with POI, and the mechanism may be related to the improvement of local inflammatory reaction. In addition, abdominal acupoints should be selected carefully.

EA at “Zusanli” (ST 36) or “Zusanli” (ST 36) combined with “Tianshu” (ST 25) can improve intestinal motility in mice with POI, and the mechanism may be related to the improvement of local inflammatory reaction. In addition, abdominal acupoints should be selected carefully.Even though the twelve meridians are connected with five zang and six fu organs separately according to the record in Huangdi Neijing (Yellow Emperor’s Internal Classic), the relations of meridians with corresponding zangfu organs are different in characteristics. Through comparing the relative characteristics between meridians and zangfu organs, it is believed that the relationship is established earlier and is quite closer between meridians and five zang organs. There is a great correlation between the disorders of five zang organs and the disorders of meridians. The disorders of five zang organs are commonly treated by the corresponding five-shu points of meridians. The relationship between meridians and six fu organs is established relatively late and the correlation between them is not as closer as that between meridians and five zang organs. The disorders of six fu organs are generally treated by the lower he-sea points. The different characteristics of the correlation between meridians and zangfu organs are relevant with the degree of their integration, the philosophical and cultrual factors and the recognition of ancient masters on zangfu organs.