This study is based on two experiments, the first, with an exploratory character. The aim of which is to assess the capacity of native vs international pop songs (NAT vs INT) from two consecutive life stages, Reminiscence bump (RB) and the immediately subsequent period (No reminiscence bump, NORB) to elicit positive emotions and autobiographical memories.

A total of 15 middle-aged adults and 15 older adults participated in Experiment 1 (E1). Emotionality, song familiarity and associated autobiographical memories were assessed. Each participant was exposed to 20 randomly selected age-specific songs. Pre-and post-test measures of mood state were also included. Experiment 2 (E2) focused on late adulthood, using a sample of 35 persons. The experimental design was similar to that used in E1. However, this second experiment also included an analysis of the types of autobiographical memories generated by the experimental task and a study of their relationship with the characteristics of the songs, their familiarculturation can be an important mediating factor in emotionality and memory. Finally, experimental design improves specific memory and positivity.The current novel coronavirus (nCoV) pandemic, COVID-19, was first reported in December 2019 in Wuhan, China, and has spread globally, causing startling loss of life, stalling the global economy, and disrupting social life. One of the challenges to contain COVID-19 is convincing people to adopt personal hygiene, social distancing, and self-quarantine practices that are related to knowledge, attitudes, and practices (KAP) of the residents of respective countries. Bangladesh, a densely populated country with a fast-growing economy and moderate literacy rate, has shown many hiccups in its efforts to implement COVID-19 policies. Understanding KAP may help policy makers produce informed decisions. This study assessed KAP in relation to COVID-19 in Bangladesh. An online survey using a pre-tested questionnaire conducted in late March 2020 attained 1,837 responses across Bangladesh. Ultimately, 1,589 completed responses were included in a statistical analysis to calculate KAP scores and their interrelations with sociodemographic variables. The overall KAP was poor, with only 33% of the participants demonstrating good knowledge, whereas 52.4% and 44.8% of the subjects showed good attitudes and practices, respectively. Sociodemographic factors had strong bearings on the KAP scores. Significantly higher KAP scores were evident in females over males, among aged 45 years and older over younger participants, and among retired workers and homemakers over students and public service employees. This study indicated a panic fuelled by poor understanding of COVID-19 associated facts and the need for the government to ensure more granular and targeted awareness campaigns in a transparent and factual manner to foster public confidence and ensure more meaningful public participation in mitigation measures. This study provides a KAP baseline regarding COVID-19 among Bangladeshis.DNA assembly is an integral part of modern synthetic biology, as intricate genetic engineering projects require robust molecular cloning workflows. Golden Gate assembly is a frequently employed DNA assembly methodology that utilizes a Type IIS restriction enzyme and a DNA ligase to generate recombinant DNA constructs from smaller DNA fragments. selleck compound However, the utility of this methodology has been limited by a lack of resources to guide experimental design. For example, selection of the DNA sequences at fusion sites between fragments is based on broad assembly guidelines or pre-vetted sets of junctions, rather than being customized for a particular application or cloning project. To facilitate the design of robust assembly reactions, we developed a high-throughput DNA sequencing assay to examine reaction outcomes of Golden Gate assembly with T4 DNA ligase and the most commonly used Type IIS restriction enzymes that generate three-base and four-base overhangs. Next, we incorporated these findings into a suite of webtools that design assembly reactions using the experimental data. These webtools can be used to create customized assemblies from a target DNA sequence or a desired number of fragments. Lastly, we demonstrate how using these tools expands the limits of current assembly systems by carrying out one-pot assemblies of up to 35 DNA fragments. Full implementation of the tools developed here enables direct expansion of existing assembly standards for modular cloning systems (e.g. MoClo) as well as the formation of robust new high-fidelity standards.Large-scale marine protected areas (LSMPAs), MPAs greater than 100,000km2, have proliferated in the past decade. However, the value of LSMPAs as conservation tools is debated, in both global scientific and policy venues as well as in particular sites. To add nuance and more diverse voices to this debate, this research examines the perspectives of stakeholders directly engaged with LSMPAs. We conducted a Q Method study with forty LSMPA stakeholders at five sites, including three established LSMPAs (the Marianas Trench Marine National Monument, United States; the Phoenix Islands Protected Area, Kiribati; the National Marine Sanctuary, Palau) and two sites where LSMPAs had been proposed at the time of research (Bermuda and Rapa Nui (Easter Island), Chile). The analysis reveals five distinct viewpoints of LSMPAs. These include three more optimistic views of LSMPAs we have named Enthusiast, Purist, and Relativist. It also depicts two more cautious views of LSMPAs, which we have named Critic and Skeptic. The findings demonstrate the multi-dimensionality of stakeholder viewpoints on LSMPAs. These shared viewpoints have implications for the global LSMPA debate and LSMPA decision-makers, including highlighting the need to focus on LSMPA consultation processes. Better understanding of these viewpoints, including stakeholder beliefs, perspectives, values and concerns, may help to facilitate more nuanced dialogue amongst LSMPA stakeholders and, in turn, promote better governance of LSMPAs.Coronavirus disease 2019 (COVID-19), the respiratory disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), was first identified in Wuhan, China and has since become pandemic. In response to the first cases identified in the United States, close contacts of confirmed COVID-19 cases were investigated to enable early identification and isolation of additional cases and to learn more about risk factors for transmission. Close contacts of nine early travel-related cases in the United States were identified and monitored daily for development of symptoms (active monitoring). Selected close contacts (including those with exposures categorized as higher risk) were targeted for collection of additional exposure information and respiratory samples. Respiratory samples were tested for SARS-CoV-2 by real-time reverse transcription polymerase chain reaction at the Centers for Disease Control and Prevention. Four hundred four close contacts were actively monitored in the jurisdictions that managed the travel-related cases.