001), whilst GFAP+ stellate astrocytes significantly decreased (p less then 0.001). Cellular reactions could be grouped into two distinct patterns an immune response associated with MHC-II/IBA1 expression and CD astrocytes; and a more innate response characterised by CD68 expression associated with WML. White matter neuroinflammation showed weak relationships to the measures of SVD, but not to the measures of AD neuropathology. In conclusion, glial responses vary extensively across the PARWM with diverse patterns of white matter neuroinflammation. Although these findings support a role for vascular factors in the pathogenesis of age-related white matter neuroinflammation, additional factors other than SVD and AD pathology may drive this. Understanding the heterogeneity in white matter neuroinflammation will be important for the therapeutic targeting of age-associated white matter damage.Permafrost thaw could trigger the release of greenhouse gases through microbial decomposition of the large quantities of carbon (C) stored within frozen soils. However, accurate evaluation of soil C emissions from thawing permafrost is still a big challenge, partly due to our inadequate understanding about the response of microbial communities and their linkage with soil C release upon permafrost thaw. Based on a large-scale permafrost sampling across 24 sites on the Tibetan Plateau, we employed meta-genomic technologies (GeoChip and Illumina MiSeq sequencing) to explore the impacts of permafrost thaw (permafrost samples were incubated for 11 days at 5°C) on microbial taxonomic and functional communities, and then conducted a laboratory incubation to investigate the linkage of microbial taxonomic and functional diversity with soil C release after permafrost thaw. We found that bacterial and fungal α diversity decreased, but functional gene diversity and the normalized relative abundance of C degradation genes increased after permafrost thaw, reflecting the rapid microbial response to permafrost thaw. Moreover, both the microbial taxonomic and functional community structures differed between the thawed permafrost and formerly frozen soils. Furthermore, soil C release rate over five month incubation was associated with microbial functional diversity and C degradation gene abundances. By contrast, neither microbial taxonomic diversity nor community structure exhibited any significant effects on soil C release over the incubation period. These findings demonstrate that permafrost thaw could accelerate C emissions by altering the function potentials of microbial communities rather than taxonomic diversity, highlighting the crucial role of microbial functional genes in mediating the responses of permafrost C cycle to climate warming.Exploration practices for oil sands developments in the boreal forest of western Canada create a network of thousands of kilometers of linear features, particularly seismic lines that dissect these forests posing significant environmental challenges. As wildfire is one of the prevalent stand-replacing natural disturbances in the Canadian boreal forest, it is an important driver of environmental change and stand development that may contribute to the mitigation of such linear industrial footprint. Here, we evaluate the short-term cumulative (also known as combined) effects of seismic lines and wildfire on biodiversity and site conditions. One year after the Horse River (Fort McMurray, Alberta, Canada) fire event in the spring of 2016, we compared dissected and undisturbed forests in burned and unburned boreal peatlands, assessing changes in overall stand structure and the responses of a variety of organisms. Soil moisture was significantly higher on seismic lines than in the adjacent forest, suggesting why most of the study sites within the fire perimeter showed little evidence of burning at the line in relation to the adjacent forest. Low fire severity on seismic lines seemed an important driver of local species diversity for ants, beetles, spiders, and plants in disturbed peatlands, resulting in similar species composition on seismic lines both within and outside the burned area, but different assemblages in burned and unburned adjacent forests. Our results suggest that fire did not erase seismic lines; rather, wildfire might increase the influence of this footprint on the recovering adjacent forest. Longer-term monitoring will be necessary to understand how boreal treed peatlands respond to the cumulative effect of wildfire and linear disturbances.

To examine the relationship between the dimensions of evidence-based practice and the therapeutic relationship and to predict the quality of the therapeutic relationship from these dimensions among nurses working in mental health units.

A cross-sectional design.

Data were collected between February-April 2018 via an online form completed by nurses working at 18 mental health units. Multiple linear regressions were used to examine the relationship between the dimensions of evidence-based practice and therapeutic relationship. Questionnaires were completed by 198 nurses.

Higher levels of evidence-based practice were a significant predictor of a higher-quality therapeutic relationship (β 2.276; 95% CI 1.30-3.25). The evidence-based practice factor which most influenced an improved therapeutic relationship was the nurses’ attitude (β 2.047; 95% CI 0.88-3.21). this website The therapeutic relationship dimension which was most conditioned by evidence-based practice dimensions was agreement on tasks, which was most favoueutic relationship in mental health nursing. This study demonstrates that an improved attitude and knowledge of evidence-based practices of mental health nurses increases shared decision-making with patients, which is a basic requirement for person-centred care. Because the therapeutic relationship is considered the backbone of nursing practice in mental health units, this research will have an impact on both mental health nurses and mental health unit managers.

We analyzed center-level outcome correlations between valve surgery and coronary artery bypass graft (CABG) in New York (NY) State and how volume-outcome effect differ between case types.

We used the 2014-2016 NY cardiac surgery outcomes report. Center-level observed to-expected (observed-to-expected ratio [O/E]) ratio for operative mortality provided risk-adjusted operative outcomes for isolated CABG and valve operations. Correlation coefficient characterized the concordance in center-level outcomes in CABG and valve. Discordant outcomes were defined as having O/E ratio greater than 2 in one operation type with O/E ratio≤1 in another. Linearized slope of volume-outcome effect in case types offered insights into centers with discordant performances between procedures.

Among 37 NY centers, annual center volumes were 220 ± 120 cases for CABG and 190 ± 178 cases for valve operations. Modest center-level correlation between CABG and valve O/E ratio was shown (R

 = 0.31). Two centers had discordant performance between valve and CABG (O/E ≤ 1 for CABG while O/E > 2 for valve procedures).