PURPOSE To determine whether there is an association between external match load and contextual factors on subjective wellness in the days before and after Australian Rules football match play. METHODS A total of 34 elite male Australian football players completed a subjective wellness questionnaire in the days leading into the match (-3, -2, and -1 d), the day of (match day), and the days after each match (+3, +2, and +1 d). Players subjectively rated each item (mood, energy, stress, leg heaviness, muscle soreness, sleep quality, hours slept, and total wellness [a sum of the total response score]) on a visual analog scale ranging from 1 to 10, with 1 representing the negative end of the continuum. External load during competitive matches was quantified using accelerometer-derived PlayerLoad, and running activity was quantified using global positioning system technology across 2 competitive seasons. The relationships between perceptions of wellness (within-individual z score), external match load, and contextual factors (match result, match location, and between-matches recovery duration) were analyzed using linear mixed models. RESULTS Mixed-effect linear models revealed trivial effects of match-day wellness z score on subsequent external match load metrics. Match result (win) and PlayerLoad in the anteroposterior vector (au·min-1) were associated with an increased (estimate ± SE 0.30 ± 0.13 z score) and reduced subjective wellness (-0.15 ± 0.06 z score), respectively. CONCLUSION The results of this study suggest that prematch perceived wellness does not relate to external match load in elite Australian football players. The between-matches microcycle length appears to be sufficient to restore perceived wellness to values that do not affect the subsequent external match loads.Hookworms cause a major neglected tropical disease, occurring after larvae penetrate the host skin. Neutrophils are phagocytes that kill large pathogens by releasing neutrophil extracellular traps (NETs), but whether they target hookworms during skin infection is unknown. Using a murine hookworm, Nippostrongylus brasiliensis, we observed neutrophils being rapidly recruited and deploying NETs around skin-penetrating larvae. Neutrophils depletion or NET inhibition altered larvae behavior and enhanced the number of adult worms following murine infection. Nevertheless, larvae were able to mitigate the effect of NETs by secreting a deoxyribonuclease (Nb-DNase II) to degrade the DNA backbone. Critically, neutrophils were able to kill larvae in vitro, which was enhanced by neutralizing Nb-DNase II. see more Homologs of Nb-DNase II are present in other nematodes, including the human hookworm, Necator americanus, which also evaded NETs in vitro. These findings highlight the importance of neutrophils in hookworm infection and a potential conserved mechanism of immune evasion. Crown All rights reserved.Evolution of antibody repertoire against the Ebola virus (EBOV) proteome was characterized in an acutely infected patient receiving supportive care alone to elucidate virus-host interactions over time. Differential kinetics are observed for IgM-IgG-IgA epitope diversity, antibody binding, and affinity maturation to EBOV proteins. During acute illness, antibodies predominate to VP40 and glycoprotein (GP). At day 13 of clinical illness, a marked increase in antibody titers to most EBOV proteins and affinity maturation to GP is associated with rapid decline in viral replication and illness severity. At one year, despite undetectable virus, a diverse IgM repertoire against VP40 and GP epitopes is observed suggesting occult viral persistence. Rabbit immunization experiments identify key immunodominant sites of GP, while challenge studies in mice found these epitopes induce EBOV-neutralizing antibodies and protect against lethal EBOV challenge. This study reveals markers of viral persistence and provides promising approaches for development and evaluation of vaccines and therapeutics. Published by Elsevier Inc.Stunting, a severe and multigenerational growth impairment, globally affects 22% of children under the age of 5 years. Stunted children have altered gut bacterial communities with higher proportions of Proteobacteria, a phylum with several known human pathogens. Despite the links between an altered gut microbiota and stunting, the role of bacteriophages, highly abundant bacterial viruses, is unknown. Here, we describe the gut bacterial and bacteriophage communities of Bangladeshi stunted children younger than 38 months. We show that these children harbor distinct gut bacteriophages relative to their non-stunted counterparts. In vitro, these gut bacteriophages are infectious and can regulate bacterial abundance and composition in an age-specific manner, highlighting their possible role in the pathophysiology of child stunting. Specifically, Proteobacteria from non-stunted children increased in the presence of phages from younger stunted children, suggesting that phages could contribute to the bacterial community changes observed in child stunting. Biological rhythms appear to be an elegant solution to the challenge of coordinating activities with the consequences of the Earth’s daily and seasonal rotation. The genes and molecular mechanisms underpinning circadian clocks in multicellular organisms are well understood. In contrast, the regulatory mechanisms and fitness consequences of biological rhythms exhibited by parasites remain mysterious. Here, we explore how periodicity in parasite traits is generated and why daily rhythms matter for parasite fitness. We focus on malaria (Plasmodium) parasites which exhibit developmental rhythms during replication in the mammalian host’s blood and in transmission to vectors. Rhythmic in-host parasite replication is responsible for eliciting inflammatory responses, the severity of disease symptoms, and fueling transmission, as well as conferring tolerance to anti-parasite drugs. Thus, understanding both how and why the timing and synchrony of parasites are connected to the daily rhythms of hosts and vectors may make treatment more effective and less toxic to hosts.