The mean SR was 1.8 ± 0.7 thus indicating an increased stiffness of the uterine scar compared to the surrounding myometrium. No significant differences were found in terms of SR according to presence of previous VD, CS during labor, type of suture or pyrexia during post-partum period. Strain Ratio was not correlated to maternal characteristics nor to labor and neonatal outcome. CONCLUSIONS Evaluation of uterine scar stiffness is feasible by using elastography. The stiffness of the uterine scar is higher than that of the surrounding myometrium and is not correlated to maternal and labor characteristics. OBJECTIVE In Taiwan (my country), the proportion of people 65 years or older is over 14% in 2018, which is known as entering “aged society”. More and more thoracolumbar burst fractures in the setting of osteoporosis happen nowadays. In this study, a finite element (FE) model on thoracolumbar burst fracture was established, and four types of posterior short-segment fixations were tested under normal bone quality and osteoporotic conditions. METHODS The intact T11-L1 spine FE model was created, and one-half of the spongy bone of the T12 vertebra was removed to simulate burst fracture. Four fixation models with posterior fusion devices were established 1. a link (S-L); 2. intermediate bilateral screws (S-I); 3. a link and calcium sulfate cement (S-L-C); 4. intermediate bilateral screws and calcium sulfate cement (S-I-C). The Young’s modulus of the osteoporotic cancellous bone was set at 70 Mpa. The range of motion (ROM), as well as the maximum value and distribution of the implant stress on T11 and L1 were compared between normal bone and osteoporotic status. RESULTS The strongest construct was S-I-C group of both normal bone and osteoporosis condition. In osteoporotic status, the ROM of construct in four types would be increased when comparing to normal bone. The stress on pedicle screws at T11 and L1 level would also be increased in osteoporosis. The value of the maximal von Mises stress on the superior vertebral body (T11) for all loading conditions was larger than that on the inferior vertebral body (L1) in both normal bone and osteoporosis. CONCLUSIONS The S-I-C provided the strongest construct even in osteoporosis status. But osteoporosis would result in weakness for spinal construct which might lead to implant failure. BACKGROUND AND AIMS TRPA1 is a calcium permeable non-selective cation channel, its expression is up-regulated in atherosclerosis plaque, yet its function in macrophages activation is unknown. We sought to establish the role of TRPA1 in inflammatory macrophages activation. METHODS TRPA1-/-ApoE-/- mice and C57BL/6 J control were treated with a high-fat diet (HFD) and the TRPA1 agonist cinnamaldehyde (CIN). Third-order branches of superior aorta of patients and mice were collected. Oil Red O staining and hematoxylin and eosin staining were performed to measure atherosclerotic lesions. The RNA-seq was performed to identify TRPA1 function in atherosclerosis. The expression of bone marrow-derived macrophages (BMDMs) markers was tested by Western blot. In addition, the levels of inflammatory factors were checked by ELISA. Chromatin immunoprecipitation (ChIP)-PCR and luciferase reporter gene assays were used to explore if TRPA1 could regulate histone modifications. RESULTS TRPA1-/-ApoE-/- mice showed a significant increase in atherosclerosis plaques compared to ApoE-/- mice after HFD treatment. Conversely, activation of TRPA1 by CIN sharply reduced atherosclerosis progression. Atherosclerosis was associated with a significant change in macrophage polarization toward the M1 proinflammatory phenotype. We found that inhibition of TRPA1 remarkably stimulated M1 marker genes expression, while repressed M2 marker genes expression. The interaction between TRPA1 and Ezh2, a subunit of polycomb repressive complex 2, suppressed the proteasome-dependent degradation of Ezh2. Thus, TRPA1 epigenetically regulated H3K27 trimethylation level in macrophages. CONCLUSIONS Our results demonstrate that TRPA1, up-regulated in atherosclerosis plaque, could regulate the macrophages toward an inflammatory phenotype, thereby modulating atherosclerosis progression. Activation of TRPA1 might serve as an atherosclerosis therapeutic target. Immobilized cells (ICs) have been widely used to enhance the remediation of organic-contaminated soil (e.g., polycyclic aromatic hydrocarbons, PAHs). Once ICs are added to the heterogeneous soil, degradation hotspots are immediately formed near the carrier, leaving the remaining soil lack of degrading bacteria. Therefore, it remains unclear how ICs efficiently utilize PAHs in soil. In this study, the viability of Silica-IC (Cells@Sawdust@Silica) and the distribution of inoculated ICs and phenanthrene (Phe) in a slurry system (soil to water ratio 12) were investigated to explore the removal mechanism of PAHs by the ICs. Results showed that the Silica-IC maintained (i) good reproductive ability (displayed by the growth curve in soil and water phase), (ii) excellent stability, which was identified by the ratio of colony forming units in the soil phase to the water phase, the difference between the colony number and the DNA copies, and characteristics of the biomaterial observed by the FESEM, and (iii) high metabolic activity (the removal percentages of Phe in soil by the ICs were more than 95% after 48 h). Finally, the possible pathways for the ICs to efficiently utilize Phe in soil are proposed based on the distribution and correlation of Phe and ICs between the soil and water phase. The adsorption-degradation process was dominant, i.e., the enhanced degradation occurred between the ICs and carrier-adsorbed Phe. This study provided new insights on developing a bio-material for efficient bio-remediation of PAHs-contaminated soil. Turning biomass into biochar as a multifunctional carbon-based material for water remediation has attracted much research attention. Sawdust and rice husk were selected as feedstock for biochar (BC) production, aiming to explore their performance as a catalyst to activate persulfate (PS) for degrading acid orange 7 (AO7). There was an excellent synergistic effect in the combined BC/PS system. Sawdust biochar (MX) showed a faster and more efficient performance for the AO7 degradation due to its abundant oxygen functional groups, compared to rice husk biochar (DK). In the BC/PS system, AO7 was well decolorized and mineralized. find more Based on the two-dimensional correlation analysis method, the azo conjugation structure and naphthalene ring of AO7 molecule changed first then benzene ring changed during the reaction. Moreover, AO7 decolorization efficiency increased with the increase of PS concentration and biochar dosage, and the deacrease of pH. Biochar deactivated after used twice. When the biochar reached its adsorption equilibrium of AO7, the AO7 could not be degraded in the BC/PS system.