6±2.5 (range 1.5 to 13.4). Follow-up was conducted by clinic. The long-term survival rate was calculated by Kaplan-Meier survival curve. Results In-hospital mortality was 16.7% (4/24), including 1 patient for multiple organ failure and 3 patients for low cardiac output syndrome (LCOS). One patient needed continuous renal replacement therapy and 6 patients suffered from LCOS. The follow-up time was 1 to 19 years, with a median of 8 years. During the follow-up period, 4 patients died, including 2 deaths for cardiogenic cause, 1 death for anticoagulant complications, and 1 death for lung cancer. The 1, 5 and 10-year survival rates were 76.2%, 71.4% and 64.9%, respectively. Conclusion The short-term and long-term clinical outcomes of TVR in patients with left ventricular dysfunction are acceptable, but the mortality and morbidity are still high.Objective To examine the clinical experience and outcomes of coronary artery bypass grafting (CABG) using radial artery as the second arterial graft. Methods Totally 585 patients in whom both left internal thoracic artery and radial artery as arterial conduits were used in CABG in Department of Cardiovascular Surgery, Nanjing Hospital Affiliated to Nanjing Medical University from April 2008 to August 2019 were consecutively enrolled. There were 436 males and 149 females, aging (63±10) years (range 36 to 86 years). There were 40.7% (238/585) of patients had diabetes and 75.6% (442/585) of them had multivessel disease (two-vessel or three-vessel diseases). From January 2017, transit time flow measurement was performed on every patient. Demographic and perioperative data were retrospectively collected, as well as follow-up data for patients who underwent CABG from January 2014 to August 2019. Analysis were made on their early and late outcomes. Results 81.9%(479/585) Most patients in this cohort (81.9%) receivedre two noncardiac death. No patient had myocardial infarction or to receive myocardial revascularization. Conclusions Radial artery as the second arterial conduit is a safe and effective strategy for CABG. Good selection of target vessel and intraoperative transit-time flow measurement may help achieve good patency, as well as the short and mid-term outcome.Objective To investigate the feasibility and effectiveness of percutaneous transhepatic cholangioscopy(PTCS) in the treatment of bilioenteric anastomotic stricture after choledochojejunostomy. Methods From April 2016 to April 2020, the clinical data of 9 patients (7 males and 2 females, aged 40-76 years) who underwent percutaneous transhepatic cholangioscopy(PTCS) for stricture expansion and lithotomy at Department of Hepatobiliary and Pancreatic Surgery, Second Affiliated Hospital of Zhejiang University School of Medicine were retrospectively analyzed. The operation was divided into two stages. In the first stage, ultrasound-guided percutaneous intrahepatic bile duct puncture was performed, and the sheath tube was inserted and fixed. In the second stage, percutaneous choledochoscopy was used for anastomotic stricture after sinus formation.The clinical outcome was evaluated by related biochemical indexes. Results The operation time was (53.3±31.0)minutes(range15-120 minutes).The postoperative hospital stay was (4.4±2.3)days(range2-9 days).After systematic treatment, the preoperative symptoms, such as abdominal pain, jaundice, fever and shivering, disappeared in 8 patients. Honokiol The range of alkaline phosphatase was 122-1 334 U/L before operation and 85-702 U/L after operation. The range of gamma glutamyl transpeptidase was 44-1 219 U/L before operation and 46-529 U/L after operation. Conclusion PTCS is a safe and effective option for minimally invasive treatment of bilioenteric anastomotic stricture.Objective To analyze the related factors of bile duct infection in patients with biliary dilatation in the short and long term after operation. Methods The data of the patients with biliary dilatation admitted in Peking Union Medical College Hospital between May 2012 and October 2020 were analyzed retrospectively. A total of 121 patients were enrolled in the study. There were 21 males(17.4%) and 100 females(82.6%),with age of (40.5±15.3) years(range 18 to 80 years). Short-term infection was defined as bile duct infection occured within 30 days after operaion; long-term infection was defined as bile duct infectiion occured 30 days after operation The relationship between bile duct infection and clinicopathologic biliary dilatation features were analyzed using univariate and multivariate analyses. Results There were 85 (70.2%),1 (0.8%), 32 (26.4%) and 3 (2.5%) patients with Todani type Ⅰ,Ⅲ,Ⅳ and Ⅴ, respectively. The mean follow-up time was (55.2±28.6) months (range 1 to 101 months). There were 18 and 37 patientinfection after biliary dilatation operation. Conclusions Hilar anastomosis and preoperative history of bile duct infection are independent related factors for short-term bile duct infection after biliary dilatation operation,and ≥45 years old and postoperative complication are independent related factors for long-term bile duct infection after biliary dilatation operation.Objective To explore the difficulties and surgical decision of laparoscopic technique in patients with complicated hepatolithiasis. Methods The clinical data of 13 patients with complicated hepatolithiasis who underwent laparoscopic hepatectomy at Department of Hepatobiliary Surgery, Affiliated Hospital of North Sichuan Medical College from December 2019 to December 2020 were collected. There were 3 males and 10 females with average age of 50.8 years (range 14 to 67 years). All patients had upper abdominal pain and a history of cholecystectomy, 4 of them had fever.Seven cases underwent laparoscopic left hemihepatectomy+bile duct exploration, 2 cases underwent laparoscopic right hemihepatectomy+bile duct exploration, 2 cases underwent laparoscopic quadrate hepatectomy (liver 4B+Part 5)+hilar cholangioplasty+bile duct exploration, 2 cases underwent laparoscopic quadrate lobe resection (liver 4B+Part 5)+cholangioplasty+cholangiojejunostomy. All patients were re-examined with abdominal ultrasound and choledochoscope 3 months after operation.