A One Health perspective must be implemented in order to develop epidemiological surveillance and establish disease control mechanisms to limit zoonotic transmission. Moreover, research in this field is important to better understand SARS-CoV-2 and to obtain the long-awaited vaccine and specific treatment.Because the use of conventional digital palpation technique for the identification of cricothyroid membrane (CTM) has been widely believed to be unreliable, the ‘laryngeal handshake’ technique (LH) has been introduced for CTM identification in the event of cricothyroidotomy. To provide evidence for clinical practice, this pilot meta-analysis aimed at investigating whether identification of CTM with the LH is superior to that with the palpation technique. SKF 14463 Studies that evaluated the accuracy of CTM identification by using LH or palpation techniques (i.e., LH group vs. Palpation group) were identified from electronic databases including PubMed, Embase, Medline, google scholar, Cochrane Central Register of Controlled Trials from inception to July 5, 2020. The primary outcome was the accuracy of both techniques. Four studies published from 2018 to 2020 were considered relevant and were read in full. We found no significant difference in success rate of CTM identification [Risk Ratio (RR) 1.09, 95% CI 0.89-1.34, p = 0.41] between the two groups. These preliminary results of the current study demonstrated no significant differences in success rate between the laryngeal handshake and conventional palpation techniques in cricothyroid membrane identification.

The long noncoding RNA (lncRNA) nuclear-enriched abundant transcript 1 (NEAT1) has been implicated in many tumors risk including gastric cancer. However, the association of single nucleotide polymorphisms (SNPs) at NEAT1 with gastric cancer risk has not been studied. The aim of this study was to investigate the association between SNPs in NEAT1 and gastric cancer susceptibility.

In this study, four SNPs in lncRNA NEAT1 were selected for genotyping in 484 gastric cancer patients and 484 controls in Chinese Han population. Quantitative real-time PCR (qRT-PCR) was conducted to evaluate the potential function of rs3825071. Attributable risk percentage (ARP) and population attributable risk percentage (PARP) were used to assess the epidemiological effect.

In the dominant model (GG), the genotypes AG + AA of rs3825071 and rs7943779 were associated with an increased risk of gastric cancer (OR = 1.72, 95%CI = 1.27-2.32 and OR = 1.63, 95%CI = 1.19-2.22). Individuals harboring ≥ 3 risk alleles have higher risk of gastric cancer (OR = 1.88, 95% CI = 1.26-2.80, P = 0.002). ARP and PARP associated with gastric cancer were 42.53% and 10.88% for rs3825071, and were 33.78% and 6.26% for rs7943779, respectively. Furthermore, compared with the genotype GG of rs3825071, the genotypes AG and AA had higher expression of NEAT1.

We found that the genetic variations in NEAT1 were significantly associated with risk of gastric cancer. The G > A variant of rs3825071 may confer gastric cancer susceptibility by changed biological effects to increase the expression of NEAT1.

 A variant of rs3825071 may confer gastric cancer susceptibility by changed biological effects to increase the expression of NEAT1.

Recent publications suggested that the risk for pre-eclampsia (PE) is higher with frozen-thawed embryo transfers (FETs) compared to fresh transfers (IVF-ETs). These studies were based on old data that reflects outdated practices. In this paper, we wanted to assess the incidence of PE in current assisted reproductive technology (ART) practice.

In this cohort study, we present the incidence of PE in all births in the province of Ontario, Canada, for the years 2013-2017 for FET, IVF-ET, and natural conceptions (NC). We also compare our findings to previous studies in a meta-analysis that includes over 4 million births.

The results of our study show that contemporary practice of ART results in comparable risk for PE between FET and IVF-ET; however, the risk is higher than NC.

Current ART practice is associated with a lower risk for PE in frozen embryo transfer; this RR can be further attenuated by using ovulatory endometrial preparation for FETs.

Current ART practice is associated with a lower risk for PE in frozen embryo transfer; this RR can be further attenuated by using ovulatory endometrial preparation for FETs.Terrestrial dissolved organic matter (tDOM) is susceptible to microbiological and photolytic oxidations and contributes significantly to the energy flow in aquatic ecosystems. However, bacterial species actively utilizing this tDOM are still not determined. We here elucidated the microbial groups actively utilizing tDOM. We characterized sediment microbial biomass and community structure using phospholipid phosphate and phospholipid fatty acids (PLFAs) analysis, respectively, and identified metabolically active members using PLFA stable-isotope-probing. Prokaryotes comprised 61% of the streambed microbial community consisting of aerobic, facultative anaerobic and anaerobic bacteria while microeukaryotes comprised the remaining 39%. Sediments were incubated in re-circulating mesocosm chambers amended with leachate from composted 13C-labelled tulip poplar (Liriodendron tulipifera L.) tree-tissues and examined for 13C incorporation into microbial PLFAs. The structure of stream sediment microbial communities prior to and after mesocosm incubation, in both the presence and absence of 13C-labeled DOM, showed no significant differences and indicated our mesocosm-based experimental design as sufficiently robust to investigate the utilization of 13C-DOM by sediment microbial communities. After 48 h of incubation, bacterial fatty acids i150, a150, 160, 161ω9, 181ω9c, 181ω7c, 10me16 and cy190 showed increased abundance of 13C. This identified the aerobic, facultative anaerobic and anaerobic bacteria as actively utilizing the 13C-labeled DOM. A single dark 48 h incubation showed incorporation into both bacterial and microeukaryotic fatty acids (204ω6, 205ω3) suggesting that microeukaryotic predators consumed bacteria that utilized 13C-labeled DOM. Hence, our data support the hypothesis that streamwater tDOM is utilized by stream bacteria, and substantially contributes to the energy flow in aquatic ecosystems.