By better understanding the mechanisms that generate natural variation in body size and subsequent effects on the evolution of life history strategies, we gain better insight into the evolutionary and ecological impacts of insect parasitoids in tri-trophic systems. © 2020 Wilson et al.The home range size and habitat use of the blue-crowned laughingthrush (Garrulax courtoisi, hereafter BCLT), a critically endangered, subtropical, cooperative-breeding bird species in southeast China, were studied during its breeding period using radio telemetry at different sites during 3 consecutive years (2016-18, from May to June of each year). A total of 17 birds (12 males, four females, and one of unknown sex) were tagged, and a total 1515 locations (mean ± se = 89.12 ± 11.42) were obtained over 54 days of tracking. The average 100% minimum convex polygon (MCP) home range size was 10.05 ± 1.17 ha, and the estimated KDE core area (fiexed kernel density estimator, KDE) size was 7.84 ± 1.18 ha. According to the Wilcoxon rank sum tests, both the 100% MCP and KDE core area size of males did not significantly differ from those of females. There were no significant differences in the 100% MCP or KDE core area sizes of the three breeding sites. The available habitats in the breeding sites included water areas, shrubs, grass plots, woodland, residential areas, vegetable field, farmland, and sandy beaches; among them, only woodland was significantly preferred by BCLTs. Woodland (average use ratio was 45.86 ± 1.74%) was strongly preferred by BCLTs for nesting, foraging and roosting. Shrubs/grass plots (24.72 ± 3.39%) and vegetable plots (11.80 ± 1.83%) were used relatively more often than the other habitats, except woodland, since shrubs were always used as perches, and vegetable plots were rich in food resources. Vertically, the canopy layer was used most often from April to June, but it was used most in May when the birds were hatching and brooding. This result indicates that BCLT is predominantly active in the upper strata during the breeding season. In addition, broadleaved trees within or adjacent to villages were important activity areas for the breeding birds; protection and management measures should be increased in these areas. ©2020 Liu et al.While the fundamental steps outlining myofibril formation share a similar scheme for different cell and species types, various granular details involved in the development of a functional contractile muscle are not well understood. Many studies of myofibrillogenesis focus on the protein interactions that are involved in myofibril maturation with the assumption that there is a fully formed premyofibril at the start of the process. However, there is little known regarding how the premyofibril is initially constructed. Fortunately, the protein α-actinin, which has been consistently identified throughout the maturation process, is found in premyofibrils as punctate aggregates known as z-bodies. We propose a theoretical model based on the particle swarm optimization algorithm that can explore how these α-actinin clusters form into the patterns observed experimentally. Our algorithm can produce different pattern configurations by manipulating specific parameters that can be related to α-actinin mobility and binding affinity. These patterns, which vary experimentally according to species and muscle cell type, speak to the versatility of α-actinin and demonstrate how its behavior may be altered through interactions with various regulatory, signaling, and metabolic proteins. The results of our simulations invite speculation that premyofibrils can be influenced toward developing different patterns by altering the behavior of individual α-actinin molecules, which may be linked to key differences present in different cell types. © 2020 Author(s).Since the traditional method generates biological waste, there is a significant demand for an easy, quick technique of blood type identification without contamination. In fact, individuals can be divided into four main blood groups whose antigens are available in red blood cell (RBC) membranes and the antibodies in the plasma. Here, UV-vis and photoluminescence (PL) spectroscopic methods are systematically used to find the spectra of blood typing antigens (A, B and AB) and antibodies i.e. VE-822 clinical trial A-Anti, B-Anti, AB-Anti and D reagent. The PL spectra of RBCs in different blood groups as well as the corresponding antibodies are successfully resolved for the purpose of blood typing. The unique photophysical characteristics of these biomolecules including signal intensity and peak emission wavelength in PL spectra are lucidly anticipated to accurately discriminate ABO groups. PL spectra of RBC in positive blood typing indicate larger signal and shorter emission peak wavelength corresponding to negative ones. Furthermore, the monoclonal antibody PL emissions emphasize that Anti-A benefits higher intensity and shorter peak wavelength (blue shift) than B-Anti. In the following, lucid blue shifts are obtained in terms of antibody concentrations accompanying the elevation of fluorescence signal, most likely due to the aggregation induced emission (AIE) phenomenon, quite the opposite of the aggregation-caused quenching (ACQ) that is widely observed from conventional chromophore. Those are envisaged as unique properties of each antibody to utilize in the spectral blood typing. © 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.Coherent light scattered by tissues brings structural and dynamic information, at depth, that standard imaging techniques cannot reach. Dynamics of cells or sub-cellular elements can be measured thanks to dynamic light scattering in thin samples (single scattering regime) or thanks to diffusive wave spectroscopy in thick samples (diffusion regime). Here, we address the intermediate regime and provide an analytical relationship between scattered light fluctuations and the distribution of cell displacements as a function of time. We illustrate our method by characterizing cell motility inside half millimeter thick multicellular aggregates. © 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.