Objective The diseases’ declaration is a fundamental tool in public health. It’s essential to know the magnitude of the problem and decide properly how to solve it. The active finding of cases allows us to know cases that weren’t registered by means of the passive declaration. In this study, we intend to analyze whether tuberculosis (TB) cases detected by Tuberculosis Units (TBU) by active finding are different to those reported passively by health professionals. Methods Data from the Galician Registry of Tuberculosis (SITUB) were collected analyzing the 2,753 TB cases detected between 2014 and 2018. Confidence intervals were compared and the data were analyzed with Chi square or T-Student tests as required. Results 44.67% of TB cases of were detected by TBU by active finding. A higher proportion was detected by active finding in bacilliferous, patients with positive culture, pulmonary location and alcoholism. It was lower in HIV (-) and in pediatric cases (under 15 years). Although the proportion of the type of declaration varied depending on the TBU or age, no changes were detected when segregating by TBU or in people over 15 years old. Conclusions If active finding wasn’t perform, almost half of the cases would be lost. We observed differences in the characteristics of the patients according to the way they have been detected, although we don’t know their possible cause. Therefore, the detection of cases by active finding it’s an important public health tool.The emergence of the novel ß-coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has resulted in a global pandemic of coronavirus disease 2019 (COVID-19). Clinical studies have documented that potentially severe neurological symptoms are associated with SARS-CoV-2 infection, thereby suggesting direct CNS penetration by the virus. Prior studies have demonstrated that the destructive neurological effects of rabies virus (RABV) infections are mediated by CNS transport of the virus tightly bound to the nicotinic acetylcholine receptor (nAChR). By comparison, it has been hypothesized that a similar mechanism exists to explain the multiple neurological effects of SARS-CoV-2 via binding to peripheral nAChRs followed by orthograde or retrograde transport into the CNS. Genetic engineering of the RABV has been employed to generate novel vaccines consisting of non-replicating RABV particles expressing chimeric capsid proteins containing human immunodeficiency virus 1 (HIV-1), Middle East respiratory syndrome (MERS-CoV), Ebolavirus, and hepatitis C virus (HCV) sequences. Accordingly, we present a critical discussion that integrates lessons learned from prior RABV research and vaccine development into a working model of a SARS-CoV-2 vaccine that selectively targets and neutralizes CNS penetration of a tightly bound viral nAChR complex.Lycopene (LYC) is known to protect cells from oxidative damage caused by free radicals in human tissues. In the present study, the authors designed a LYC-loaded sialic acid (SA)-conjugated poly(D,L-lactide-co-glycolide) (PLGA) nanoparticle (LYC-NP) to enhance the therapeutic efficacy of LYC in acute kidney injury. The characteristics of the LYC-NPs were defined according to particle size, morphology, and in vitro drug release. The LYC-NPs exhibited a controlled release of LYC over 48 h. Confocal laser scanning microscopy clearly highlighted the targeting potential of SA. Enhanced green fluorescence was observed for the LYC-NPs in H2O2-treated human umbilical vein endothelial cells, indicating enhanced internalisation of NPs. NCT-503 The LYC-NPs showed significantly greater cell viability than H2O2-treated cells. In addition, the LYC-NPs remarkably reduced proinflammatory cytokine levels, attributable mainly to the increased cellular internalisation of the SA-based carrier delivery system. Furthermore, protein levels of caspase-3 and -9 were significantly down-regulated after treatment with the LYC-NPs. Overall, they have demonstrated that SA-conjugated PLGA-NPs containing LYC could be used to treat kidney injury.The emergence of the huge number of multi-drug resistant (MDR) bacteria requires an alternative to the drugs. Silver nanoparticles (AgNPs) are a strong candidate for this due to their bactericidal properties, which can be better concluded by understanding their morphology and chemistry. The study hypothesised that AgNPs synthesised using leaves of Syzygium cumini can be used to treat locally emerging MDRs forming biofilms on indwelling medical devices. Synthesised particles were characterised by methods like UV-visible spectroscopy, X-ray powder diffraction, scanning electron microscopy, transmission electron microscopy, and Zetasizer. Fourier transform infrared spectroscopy, and high-performance liquid chromatography were used to predict phytochemicals present in the leaves. The shape of particles is revealed to be relatively spherical, with average size to be around 10-100 nm. Phenolic compounds are attributed to the formation of nanoparticles, stability analysis shows particles to be stable, and zeta potential determined the surface charge to be -20.1 mV. Biosynthesised particles are found to possess efficient antibacterial activity MDR bacteria developing biofilms in medical devices; hence, it is concluded that S. cumini based NPs can be used to develop a layer on implant-related medical devices. Toxicity evaluation against A594 cancer cells portrays AgNPs to be potential tumour reduction agents in a concentration-dependent manner.Metallic nanoparticles can be synthesised in living plants, which provide a friendly approach. In this work, the authors aimed to study the synthesis of silver nanoparticles (AgNPs) in Arabidopsis and the two-dimensional (2D) distribution of Ag and other elements (Ca, P, S, Mg, and CI) in the Arabidopsis plant tissues. The concentrations of Ag in the plant tissues were determined by inductively coupled plasma-atomic emission spectrometer, showing that the majority of Ag was retained in the roots. Transmission electron micrographs showed the morphology of AgNPs and the location in plant cells. The distributions of Cl and Ag were consistent in plant tissues by 2D proton-induced X-ray emission. In conclusion, this is the first report of the AgNP synthesis in Arabidopsis living plants and its 2D distribution of important elements, which provide a new clue for further research.