The sparse non-negative matrix under-approximation (SNMU) was used to identify mixtures of chemical substances. A k-means clustering classification of the whole study population was then performed to define clusters with similar co-exposure profiles. Overall, 8 mixtures which explained 83% of the total variance, were retained. The first mixture, entitled “Minerals, inorganic contaminants, and furans”, explained the highest proportion of the total variance (38%), and was correlated in particular with the consumption of “Offal” (rho = 0.22), “Vegetables except roots” (rho = 0.20), and “Eggs” (rho = 0.19). The other seven mixtures explained between 17% and 1% of the variance. Finally, 5 clusters were identified based on the adherence to the 8 mixtures. This study, being the largest ever conducted to identify dietary exposure to chemical mixtures, represents a concrete attempt to prioritize mixtures for which it is essential to investigate combined health effects based on exposure.

Chlordecone (CD), also known as Kepone, is an organochlorine insecticide that has been used in banana crops in the French West Indies. Due to long-term contamination of soils and water, the population is still exposed to CD. Exposure to CD in adulthood is associated with an increased risk of prostate cancer (PCa).

We examined the transgenerational effects of CD on murine prostate tissue.

We exposed pregnant Swiss mice to CD. The prostates from directly exposed (F1) and non-exposed (F3) male progeny were analyzed. We used immunofluorescence, RNA-seq and ChIP-seq techniques for the comprehensive analyses of chromatin states in prostate.

We observed an increased prostatic intraepithelial neoplasia phenotype (PIN) in both F1 and F3 generations. Transcriptomic analysis in CD-derived F1 and F3 prostate using RNA-seq revealed that 970 genes in F1 and 218 in F3 genes were differentially expressed. The differentially expressed genes in both datasets could be clustered accordingly to common biological processesmonal pathway and self-renewal gene expression that have the capacity to promote neoplasia in CD-exposed mice.The reduction of greenhouse gas emissions is one of the greatest global challenges through 2050. Besides greenhouse gas emissions, air pollution, such as nitrogen oxide and particulate matter emissions, has gained increasing attention in agglomerated areas with transport vehicles being one of the main sources thereof. Alternative fuels that fulfill the greenhouse gas reduction goals also offer the possibility of solving the challenge of rising urban pollution. This work focuses on the electric drive option for heavy and light duty vehicle freight transport. In this study, fuel cell-electric vehicles, battery-electric vehicles and overhead catenary line trucks were investigated, taking a closer look at their potential to reduce greenhouse gas emissions and air pollution and also considering the investment and operating costs of the required infrastructure. This work was conducted using a bottom-up transport model for the federal state of North Rhine-Westphalia in Germany. Two scenarios for reducing these emissic trucks. selleck Articulated trucks have the highest potential to be operated as overhead trucks. However, the results indicated that air pollution is only partially reduced by switching conventional articulated trucks to electric drive models. The overall results show that a comprehensive approach such as fuel cell-electric drives for all trucks would most likely be more beneficial.

To investigate the effects of hydration with or without Hydroxyethyl Starch (HES) 130/0.4 on neurological outcomes and medical costs during hospitalisation in patients with a single infarction (SI) in the posterior lenticulostriate artery (LSA) territory.

In this retrospective, single-centre, non-blinded cohort study, SI in the posterior LSA was defined as an ischaemic lesion with a high-signal intensity area ≥20 mm. All patients received basic stroke care within 48 h of symptom onset between April 2015 and January 2019. Patients were divided into the following two groups by clinician’s preference 1) those administered HES 130/0.4 and 2) those receiving other infusion fluid. The relationships between hospital costs and hydration therapy type were examined.

Eighteen (31%) of 58 patients received HES 130/0.4. The HES group had a significantly lower total cost than the control group (3.6 vs. 6.4 million yen, p=0.006). Moreover, the HES group had a significantly shorter hospital stay duration (79.5 vs. 141.0 days) and lower National Institutes of Health Stroke Scale score on day 7. Multivariate analysis found that HES 130/0.4 administration was an independent factor associated with high costs.

Hydration therapy with HES 130/0.4 significantly decreased the total costs and hospitalisation duration of patients with SI in the posterior LSA territory.

Hydration therapy with HES 130/0.4 significantly decreased the total costs and hospitalisation duration of patients with SI in the posterior LSA territory.Water scarcity is perceived as a global systemic risk since there is an inexorable rise in water demand. An ecological engineering system mimics a natural ecosystem by balancing the trophic conditions for effective treatment of wastewater in a sequential manner. The present study was designed using emergent, floating and submerged macrophytic plants in a systematic approach. The study was evaluated with several components such as plants (water hyacinth, water lettuce and water thymes), aeration (supply of oxygen), and physical adsorption (activated carbon). Domestic wastewater collected from the local effluent treatment plant was treated individually and by combining all the components. Diverse experimental setups viz., lake sediment (control reactor), aeration, activated carbon blocks, water hyacinth, water lettuce, and water thymes were individually studied. Further the above components were combined, such as lake sediment + aeration + activated carbon blocks with plants like water hyacinth, water lettuce, and water thymes. The study inferred along with phytoremediation, and the external factors enhanced the treatment performances. Water hyacinth documented enhanced chemical oxygen demand removal efficiency of 85.71%, followed by water lettuce (80%), and water thymes (77.14%) along with the plants, both aeration, and activated carbon had stimulated the wastewater treatment. The highest removal efficiency of nitrate (70.23%), phosphate (63.64%), and sulphate (61.16%) were observed in water hyacinth due to its thick roots, and fibrous tissues reported effective treatment. The study hypothesized that these processes could be an effective strategy to restore the lakes and regulate the environmental flow. The study infers that an ecological engineering system symbiotically enables to self-organize the ecosystem within the boundary.