OBJECTIVE Chemerin concentrations are elevated in obesity and associated with inflammation and insulin resistance. Exercise improves insulin sensitivity, which may be facilitated by changes in chemerin. We explored the effects of 24 -week aerobic exercise program, diet and both combined on serum and abdominal adipose tissue (AT) chemerin, and adiponectin concentrations in obese women. METHODS Thirty volunteers participated in a 24-week intervention whereby the women were randomly categorised into three groups 1) exercise (EX), 2) diet (DI) and 3) exercise and diet group (EXD). Blood samples were taken before and after 12-week and 24-week of the intervention. Adipose Tissue (AT) biopsies were gathered only at baseline and at the end of the experiment. RESULTS We found increases in VO2max in EXD (21.8 and 39.5%, p less then 0.05) and in the EX group (18.1 and 41%, p less then 0.05) after the 12 and 24-week training, respectively. Chemerin circulating levels were reduced in EXD and DI groups, after 24-week training program (p less then 0.01). HOMA-R decreased only in the EXD group (p less then 0.05). However, circulating adiponectin increased in both EXD and DI groups after 24-week training (p less then 0.01). After 6 months training, AT biopsies showed decreases in chemerin levels in EXD and DI groups (p less then 0.01). No changes in AT biopsies were observed in EX group. Nevertheless, adiponectin increased (p less then 0.05) in AT. CONCLUSION Aerobic training and diet, whether applied exclusively or combined have an impact on circulating or AT levels of adiponectin and chemerin in to the exclusion of the EX group. Therefore The 24-week training program and diet used in this study was very effective for producing significant benefits to body composition, insulin resistance and lipids profile, as well as lowering chemerin levels in these obese women. Therefore, our data suggests that chemerin serum concentrations are associated with insulin resistance.Polycystic ovary syndrome (PCOS) as well as hyperprolactinemia can cause infertility. In retrospective study the prolactin levels during the oral metoclopramide test among lean PCOS woman according to four phenotypes and free androgen index (FAI) were compared. The study population consisted of 314 lean PCOS women. The population was divided into four groups according to the FAI and menstrual cycle regularity. The group A consisted 126 women with FAI≥5 and irregular menstruation, the group B- 53 patients with FAI≥5 and regular menstruation. Group C- 70 patients with FAI less then 5 and irregular menstruation, group D – 65 patients with FAI less then 5 and regular menstruation. The ratio of prolactin value in 120th minute in the metoclopramide test to the basal prolactin value was higher in group D than in groups A and B. The prolactin basal concentration was higher in patients with FAI≥5 than in patients with FAI less then 5, (262.9 vs 228.9 µIU/ml; p less then 0.001). The ratio of prolactin in 60th minute (12.3 vs 16.7; p=0.006) and in the 120th minute (10.9 versus 13.3; p less then 0.001) of the metoclopramide test to the basal prolactin were lower in patients with FAI≥5. The prolactin secretion in lean PCOS women may be associated with their FAI.OBJECTIVES A novel method of long-term telemetric monitoring of mean arterial pressure (MAP) and intracranial pressure (ICP) for the determination of current cerebral perfusion pressure (CPP) and the time course of ICP in freely moving rats under physiological conditions and with increased ICP due to the induced cerebral edema were studied. METHODS The brain edema, that caused volume enlargement and ICP elevation was achieved in entirely experimental conditions without any parallel pathological process. Vasogenic/extracellular edema was induced by osmotic blood-brain barrier disruption (BBBd) and for induction of cytotoxic/intracellular edema the water intoxication model (WI) was used. RESULTS The results showed significantly elevated values of ICP both in conditions of osmotic blood-brain barrier disruption (BBBd model) and cytotoxic/intracellular edema (WI model) compared to intact rats. The average values of ICP were significantly higher in WI model compared to osmotic BBBd model. Distinct pattern of elevated ICP, related to the selected way of experimental brain edema induction, was found. Oridonin nmr In the experimental model of osmotic BBB disruption, the elevation of ICP started earlier but was of very short duration. In WI model the elevation of ICP was present during the whole period of monitoring. CONCLUSION Our results indicate that purely experimental models of brain edema (WI, BBBd) without any parallel pathological process can compromise the basic brain homeostatic activity.BACKGROUND Two-dimensional speckle-tracking echocardiography (2D STE) has been demonstrated to have certain diagnostic utility in heart transplantation (HTX) patients with acute cardiac allograft rejection (ACAR). The aim of the systematic review and meta-analysis was to evaluate the diagnostic value of common strain parameters for ACAR in HTX patients. METHODS After conducting a database search, we selected studies evaluating left ventricular global longitudinal strain (GLS), circumferential strain (CS), radial strain (RS), and free wall right ventricular longitudinal strain (RV FW) in rejection group vs rejection-free group. RESULTS After reviewing 886 publications, seven studies were finally included in the meta-analysis, representing the data of 1173 pairs of endomyocardial biopsy and echocardiographic examination. Heart transplantation patients with rejection had significantly lower GLS than rejection-free subjects (weighted mean difference -2.32 (95% CI, -3.41 to -1.23; P  less then  .001). Heart transplantation patients with rejection had significantly lower CS than rejection-free subjects (weighted mean difference -2.49 (95% CI, -4.05 to -0.91; P = .0019). In addition, HTX patients with rejection also had significantly lower RV FW (weighted mean difference -4.90 (95% CI, -6.15 to -3.65; P  less then  .001). CONCLUSIONS The meta-analysis and systematic review demonstrate that myocardial strain parameters derived from 2D STE may be useful in detecting ACAR in HTX patients. The present results provide encouraging evidence to consider the routine use of GLS, CS, and RV FW as markers of graft function involvement during ACAR. © 2020 Wiley Periodicals, Inc.