27±11.53) and gain-frame (60.8±7.77, p⟨0.001) and loss-frame (60.25±8.96, p⟨0.001) groups for post-test practice scores. No difference was found between the gain-frame and loss-frame groups for the post-test scores (p=0.69). CONCLUSION Gain-frame and loss-frame SMS had similar impacts on the mothers’ knowledge and practices about their children’s oral health. However, both improved the mothers’ knowledge and practice. Therefore, regardless of the style of text message framing, this method may be useful for educating mothers. Copyright© 2020 Dennis Barber Ltd.Despite recent revisions, the classification of personality disorder remains a matter of dispute, and there is little evidence of consistent progress toward an evidence-based system. This essay examines four issues impeding taxonomic progress and explores how they might be addressed. First, the phenomenological and aetiological complexity of personality disorder poses a formidable challenge to traditional taxonomic methods. Second, current classifications incorporate assumptions such as a stringent version of medical model and an essentialist philosophy that are inconsistent with empirical evidence. Third, despite the claims of trait psychology, a viable alternative to categorical diagnosis is not available. Contemporary trait models have not gained widespread clinical acceptance and substantial conceptual and methodological limitations compromise their clinical value. Finally, the processes used to revise official classifications are biased toward conservative revisions and difficult to shield from non-scientific influences. It is suggested that rather making further attempts to develop a general monolithic classification that meets all needs, consideration be given to developing a more flexible and multifaceted framework that combines diagnosis and assessment. © 2019 John Wiley & Sons, Ltd.Scarless skin regeneration with functional tissue remains a challenge for full-thickness wounds. Here, mesenchymal stem cell (MSC)-laden hydrogels are developed for scarless wound healing with hair follicles. Microgels composed of aligned silk nanofibers are used to load MSCs to modulate the paracrine. MSC-laden microgels are dispersed into injectable silk nanofiber hydrogels, forming composites biomaterials containing the cells. The injectable hydrogels protect and stabilize the MSCs in the wounds. The synergistic action of silk-based composite hydrogels and MSCs stimulated angiogenesis and M1-M2 phenotype switching of macrophages, provides a suitable niche for functional recovery of wounds. Compared to skin defects treated with MSC-free hydrogels, the defects treated with the MSC-laden composite hydrogels heal faster and form scarless tissues with hair follicles. Wound healing can be further improved by adjusting the ratio of silk nanofibers and particles and the loaded MSCs, suggesting tunability of the system. To the best of current knowledge, this is the first time scarless skin regeneration with hair follicles based on silk material systems is reported. The improved wound healing capacity of the systems suggests future in vivo studies to compare to other biomaterial systems related to clinical goals in skin regeneration in the absence of scarring. © 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.Current standard of care dressings are unsatisfactorily inefficacious for the treatment of chronic wounds. Chronic inflammation is the primary cause of the long-term incurable nature of chronic wounds. Herein, an absorbable nanofibrous hydrogel is developed for synergistic modulation of the inflammation microenvironment to accelerate chronic diabetic wound healing. The electrospun thioether grafted hyaluronic acid nanofibers (FHHA-S/Fe) are able to form a nanofibrous hydrogel in situ on the wound bed. This hydrogel degrades and is absorbed gradually within 3 days. The grafted thioethers on HHA can scavenge the reactive oxygen species quickly in the early inflammation phase to relieve the inflammation reactions. Additionally, the HHA itself is able to promote the transformation of the gathered M1 macrophages to the M2 phenotype, thus synergistically accelerating the wound healing phase transition from inflammation to proliferation and remodeling. On the chronic diabetic wound model, the average remaining wound area after FHHA-S/Fe treatment is much smaller than both that of FHHA/Fe without grafted thioethers and the control group, especially in the early wound healing stage. Therefore, this facile dressing strategy with intrinsic dual modulation mechanisms of the wound inflammation microenvironment may act as an effective and safe treatment strategy for chronic wound management. © 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.Biological systems, which possess remarkable functions and excellent properties, are gradually becoming a source of inspiration for the fabrication of advanced tissue regeneration biomaterials due to their hierarchical structures and novel compositions. It would be meaningful to learn and transfer the characteristics of creatures to biomaterials design. However, traditional strategies cannot satisfy the design requirements of the complicated bioinspired materials for tissue regeneration. 3D printing, as a rapidly developing new technology that can accurately achieve multimaterial and multiscale fabrication, is capable of optimizing the fabrication of bioinspired materials with complex composition and structure. This review summarizes the recent developments in 3D-printed bioinspired biomaterials for multiple tissue regeneration, and especially highlights the progresses on i) traditional bioinspired designs for biomaterials fabrication, ii) biological composition inspired designs for the 3D-printed biomaterials, and iii) biological structure inspired designs for the 3D-printed biomaterials. Masitinib clinical trial Finally, the challenges and prospects for the development of 3D-printed bioinspired biomaterials are discussed. © 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.Three-dimensional organoid tissue culture models are a promising approach for the study of biological processes including diseases. Advances in these tissue culture technologies improve in vitro analysis compared to standard 2D cellular approaches and are more representative of the physiological environment. However, a major challenge associated with organoid systems stems from the laborious processing involved in the analysis of large numbers of organoids. Here the design, characterization, and application of silk-elastin-like protein-based smart carrier arrays for processing organoids is presented. Fabrication of hydrogel-based carrier systems at room temperature result in organized arrays of organoids that maintain tissue culture plate orientation and could be processed simultaneously for histology. The system works by transfer of the organoids to the hydrogel arrays after which the material is subjected to 65 °C to induce hydrogel contraction to secure the organoids, resulting in multisample constructs and allowing for placement on a microscope slide.