Intradevice measurement repeatability was excellent for both the CASIA (ICC range 0.93-0.99) and ANTERION (ICC range 0.97-0.99). Interdevice measurement agreement was also excellent (ICC range 0.85-0.96). Measurements within and between devices were similar (

> 0.06) for all parameters except SSA500 (

= 0.03). Linear regression and Bland-Altman plots showed the relationship was consistent across the entire range of measurements.

Intradevice measurement repeatability is excellent for the CASIA and ANTERION. Interdevice measurement agreement between the two devices exceeds metrics reported by previous comparison studies.

Modern swept-source AS-OCT devices produce highly repeatable measurements of ocular biometric parameters that are nearly interchangeable across devices.

Modern swept-source AS-OCT devices produce highly repeatable measurements of ocular biometric parameters that are nearly interchangeable across devices.

Lacritin is a tear glycoprotein with pro-tearing and pro-ocular surface homeostasis activities that is selectively deficient in most dry eye tears. Proteoforms include an active monomer, inactive polymers, and a splice variant termed lacritin-c. Quantitation of the different proteoforms of tear lacritin may provide a diagnostic tool for ocular diseases. Here, we report the development of an immunoassay for the quantification of multiple lacritin proteoforms in human tear samples.

Basal tears collected on Schirmer test strips with anesthesia were eluted by diffusion and centrifugation under optimized conditions. Tear protein concentrations were determined, and 2.56 µg of each sample was separated by SDS-PAGE followed by western blot analysis. Blots were challenged with anti-Pep Lac N-term antibodies. Detection was with fluorescent secondary antibodies visualized by the LI-COR Odyssey CLx imaging system and quantified with standard curves of recombinant lacritin.

The percent total lacritin (ng lacritin/100 ng total protein) ranged from 1.8% to 14.8%. Monomer, lacritin-c, and polymer proteoform percent total protein ranged from 1.1% to 6.3%, 0.3% to 5.4%, and 0.7% to 5.7%, respectively. Monomer lacritin was detected at concentrations of 6 to 176 µM, with lacritin-c and polymer proteoforms at 2 to 46 µM and 1 to 23 µM, respectively.

This assay greatly exceeds the power and sensitivity of our prior lacritin enzyme-linked immunosorbent assay that was not capable of distinguishing monomer from polymers and lacritin-c proteoforms.

A new method has been developed to quantitate multiple proteoforms of tear lacritin in preparation for analyses of samples from clinical trials.

A new method has been developed to quantitate multiple proteoforms of tear lacritin in preparation for analyses of samples from clinical trials.

Cluster trend analysis detects glaucomatous deterioration within predefined subsets (clusters) of visual field locations. However, it may miss small defects straddling boundaries between the clusters. This study assesses whether simultaneously using a second set of clusters, overlapping the first, could improve progression detection.

Deterioration in eyes with or at risk of glaucomatous visual field loss was “detected” by mean deviation (MD) on the first visit at which the

value from linear regression over time was below the fifth percentile of its permutation distribution. Similarly,

values were calculated for each of 10 predefined nonoverlapping clusters of locations, or 21 overlapping clusters; deterioration was “detected” when the

th-smallest

value was below the fifth percentile of its permutation distribution, for different

. Times to detect deterioration were compared using survival models.

Biannual series of ≥5 visual fields (mean = 14) were available for 420 eyes of 213 participants. Deterioration of 33% of eyes was detected earliest using

= 1 overlapping cluster in 3.3 years (95% confidence interval 2.7-4.6 years); or

= 2 nonoverlapping clusters in 3.3 years (2.7-5.0) (comparison

= 0.654). There was also no significant difference in the probability that deterioration would be confirmed (92.8% vs. Temsirolimus 94.4%,

= 0.289). Both overlapping and nonoverlapping clusters detected deterioration significantly sooner than MD (4.5 years,

≤ 0.001).

After equalizing specificity, overlapping clusters of locations did not significantly reduce the time to detect deterioration compared with nonoverlapping clusters.

Cluster trend analyses detected deterioration sooner than global analyses even when defects straddled cluster borders.

Cluster trend analyses detected deterioration sooner than global analyses even when defects straddled cluster borders.

To compare visual function of myopic children who had worn either defocus incorporated multiple segment (DIMS) spectacle lenses or single vision (SV) spectacle lenses over two years.

We included 160 Chinese myopic (-1 diopter [D] to -5 D) children aged 8 to 13 years in a randomized clinical trial; they wore either DIMS lenses (DIMS;

= 79) or regular SV spectacles lenses (

= 81) full time for 2 years. Visual function, including high-contrast visual acuity (VA) and low-contrast VA at distance and near, binocular functions, and accommodation, before, during, and after 2 years of spectacle wear were assessed when both groups wore SV corrections. Changes of visual function between the two groups and within groups were compared.

There were no statistically significant differences in the 2-year visual function changes between DIMS and SV groups (repeated measures analysis of variance with group as factor;

> 0.05). Statistically significant improvement in the best-corrected distance high-contrast VA (

< 0.001) and stereoacuity score (

< 0.001) were found after DIMS lens wear over 2 years. Similar findings were observed after SV spectacle lens wear. For both the DIMS and SV groups, there were statistically significant decreases in accommodative lag, monocular and binocular amplitude of accommodation after two years (

< 0.01), but not in the changes in distance low-contrast VA, near high-contrast VA, near low-contrast VA, or phoria.

Although changes in some visual function were shown during 2 years of DIMS lens wear, similar changes were found with SV lens wear. Wear of DIMS spectacle lenses for 2 years does not adversely affect major visual function when children return to SV corrections.

DIMS spectacle lenses did not cause any adverse effects on visual function.

DIMS spectacle lenses did not cause any adverse effects on visual function.