PURPOSE We have developed a new method to track tumor position using fluoroscopic images, and evaluated it using hepatocellular carcinoma case data. METHODS Our method consists of a training stage and a tracking stage. In the training stage, the model data for the positional relationship between the diaphragm and the tumor are calculated using four-dimensional computed tomography (4DCT) data. The diaphragm is detected along a straight line, which was chosen to avoid 4DCT artifact. In the tracking stage, the tumor position on the fluoroscopic images is calculated by applying the model to the diaphragm. Using data from seven liver cases, we evaluated four metrics diaphragm edge detection error, modeling error, patient setup error, and tumor tracking error. We measured tumor tracking error for the 15 fluoroscopic sequences from the cases and recorded the computation time. VPS34-IN1 RESULTS The mean positional error in diaphragm tracking was 0.57 ± 0.62 mm. The mean positional error in tumor tracking in three-dimensional (3D) space was 0.63 ± 0.30 mm by modeling error, and 0.81-2.37 mm with 1-2 mm setup error. The mean positional error in tumor tracking in the fluoroscopy sequences was 1.30 ± 0.54 mm and the mean computation time was 69.0 ± 4.6 ms and 23.2 ± 1.3 ms per frame for the training and tracking stages, respectively. CONCLUSIONS Our markerless tracking method successfully estimated tumor positions. We believe our results will be useful in increasing treatment accuracy for liver cases. BACKGROUND Developments in rehabilitation technology such as video-based exergaming contributes to the treatment process as well as to increase the active participation of persons with multiple sclerosis (pwMS). The aim was to investigate the effect of video-based exergaming training on upper extremity and cognitive function as well as core stability, walking, depression, fatigue, and quality of life in pwMS. METHODS This randomized controlled trial included 60 pwMS who were randomly divided into three groups; video-based exergaming (n = 21), conventional rehabilitation (n = 19), and control groups (n = 20). The experimental groups received therapy sessions once a week for 8 weeks. All the participants were assessed at baseline and after 8 weeks. The outcome measures included upper extremity and cognitive functions as well as core stability, walking, depression, fatigue, and quality of life measures. RESULTS Significant improvements were observed in the primary outcome, measured by Nine-Hole Peg Test in the vfe in pwMS. BACKGROUND Altered lipid metabolism is a feature of systemic autoimmune diseases. Dyslipidemia is associated with the disease activity and progression in patients with multiple sclerosis. However, in neuromyelitis optica spectrum disorder (NMOSD), changes in the lipid profile and the associations between specific lipid levels and disease activity/disability are unknown. METHODS Serum samples (N = 148) were collected from 53 patients with aquaporin-4 (AQP4)-positive NMOSD when they were not treated with lipid lowering agents. Fasting lipid (total cholesterol, triglyceride [TG], high-density lipoprotein cholesterol [HDL-C], low-density lipoprotein cholesterol) levels were compared between 39 patients with NMOSD, not taking steroids, and 142 age-, sex-, and body mass index-matched healthy controls. In addition, we analyzed the differences in the lipid profile between attack and remission samples and the associations between lipid profiles and clinical outcome in all 148 samples from 53 patients. The generalized estimating equation was used. RESULTS Patients with NMOSD showed lower HDL-C and higher TG levels compared to healthy controls (p = 0.017 and p  less then  0.001, respectively). HDL-C level was significantly lower during attack than remission (β = -7.851; p = 0.035), and TG level had positive correlation with EDSS scores (β = 0.014; p = 0.002) regardless of disease activity status. However, enhanced lesions on magnetic resonance imaging were not associated with lipid profiles. CONCLUSION Dyslipidemia with low HDL-C and high TG correlated disease activity and disability in AQP4-positive NMOSD. It remains to be elucidated whether altered lipid metabolism contributes to deleterious immune response, possibly through inflammation, or is secondary to neurological disability in NMOSD. It is an effective solution to overcome the bottlenecks of commercial production of microalgal biomass by providing cost-effective and environment-friendly organic carbon sources for microalgal mixotrophic growth. In this study, effects of lignocellulose-related mannitol and xylitol on the growth, photosynthetic pigment content, cell morphology, and metabolites biosynthesis of freshwater microalga Euglena gracilis were investigated. The results revealed that both mannitol and xylitol effectively promoted the growth of E. gracilis, and at the optimal dosage of 4 g·L-1, the biomass yield was increased by 4.64-fold and 3.18-fold, respectively. Increase of cell aspect ratio was only observed in mannitol treatment groups, indicating that E. gracilis had different physiological responses to mannitol and xylitol. Fourier transform infrared spectroscopy combined with multivariate analysis was applied to analyze the cellular components. The lipid content of E. gracilis was significantly promoted by these two sugar alcohols, which would increase its potential in biofuel production. Enhanced macroalgal biochars with large specific surface areas (up to 399 m2 g-1), partly graphitized structure, high nitrogen doping (up to 6.14%), and hydrophobicity were fabricated by co-carbonization of macroaglae, ferric chloride, and zinc chloride. These biochars were used as sorbents for the removal of polycyclic aromatic hydrocarbons from water. The sorption capacity of polycyclic aromatic hydrocarbons onto macroalgal biochars was high (up to 90 mg g-1), and recycling by thermal desorption was practicable. We revealed the physical-dominated multilayer sorption process, based on results from characterization and sorption experiments. Pore filling, mass transfer, π-π stacking, and the partition effect were found to be possible sorption mechanisms. This study suggests that porous graphitized nitrogen-doped biochars may be synthesized from macroalgae with simple one-pot carbonization and display promising applicability for sorption removal of organic pollutants from water.