A novel electrochemical biosensing strategy was proposed to detect cytokeratin fragment antigen 21-1 (CYFRA 21-1) DNA based on Exo III-assisted digestion of dsDNA polymer (EADDP) from hybridization chain reaction (HCR). Primarily, the presence of target can drive a catalytic hairpin assembly (CHA) reaction, which was aimed to achieve target recognition and circulation. Then the HCR can be triggered for further signal amplification and generate long dsDNA polymer with signal tags. Subsequently, the introduction of Exo III can digest the long dsDNA polymer to produce large amounts of double signal fragments (DSFs). The above experiments were all carried out in homogeneous solution. GSK343 Finally, the released DSF can be captured onto the electrode directly by capture probe (CP) and a highly amplified electrochemical signal can be detected. The EADDP in homogeneous solution circumvented complex solid-liquid interface reaction and tedious operation steps on electrode. Besides, one target can be converted into abundant DSFs, which greatly improved the sensitivity. This biosensor exhibited a low detection limit (0.0348 fM) and wide linear range (5 fM ∼ 50 nM) for CYFRA 21-1 DNA biosensing with reliable specificity and stability.There is a growing interest in conductivity detection for capillary electrophoresis; especially because of capacitively coupled contactless conductivity approach. This robust and general-purpose detector has another lesser-known feature sensitivity does not depend on the very chemical nature of the analyte, but only on its effective charge and effective mobility. Therefore, the calibration curve prepared for a given species may be used to quantify another one of same charge and mobility. In the absence of a species (calibrant) of exactly the same mobility, two or more calibrants can be used. Provided the sensitivity varies smoothly in the desired region of mobility, it can be mathematically described by a function. For small ranges of mobilities, a linear behavior is expected, and the sensitivity for the analyte can be obtained by interpolation. This technique was investigated for eight different combinations of mono- and double-charged cationic and anionic analytes using buffered and unbuffered background electrolytes (BGEs). For most of the applications, a linear model was enough to describe the sensitivity (0.988 less then R2 less then 0.998), but for ample range of mobilities, the inclusion of a hyperbolic term was needed (0.995 less then R2 less then 0.999). This technique has a great potential to be used in field applications and in laboratories when the analytes are unstable or they are not available to be used in the preparation of standard solutions.Prostate cancer, a leading cause of cancer-related deaths worldwide, principally occurs in over 50-year-old men. Nowadays there is urgency to discover biomarkers alternative to prostate-specific antigen, as it cannot discriminate patients with benign prostatic hyperplasia from clinically significant forms of prostatic cancer. In the present paper, 32 benign prostatic hyperplasia and 41 prostatic cancer urine samples were collected and analyzed. Polar and positively charged metabolites were therein investigated using an analytical platform comprising an up to 40-fold analyte enrichment step by graphitized carbon black solid-phase extraction, HILIC separation, and untargeted high-resolution mass spectrometry analysis. These classes of compounds are often neglected in common metabolomics experiments even though previous studies reported their significance in cancer biomarker discovery. The complex metabolomics big datasets, generated by the UHPLC-HRMS, were analyzed with the ROIMCR procedure, based on the selection of the MS regions of interest data and their analysis by the Multivariate Curve-Resolution Alternating Least Squares chemometrics method. This approach allowed the resolution and tentative identification of the metabolites differentially expressed by the two data sets. Among these, amino acids and carnitine derivatives were tentatively identified highlighting the importance of the proposed methodology for cancer biomarker research.A facile, novel fabrication approach using UV light irradiation was proposed to fabricate a photo-responsive metal-organic framework (PR-MOF-1, [Zn2-(bdc)2-(dpNDI)]n, where bdc = benzene-1,4-dicarboxylic acid; dpNDI = N,N’-di(4-pyridyl)-1,4,5,8-naphthalenetetracarboxy diimide) membrane. The morphology of the PR-MOF-1 framework evolved from a honeycomb porous structure to a densified ladder-layered structure after 60 min of UV-light illumination. The as-grown film was optically transparent and exhibited a greater sensing response to ethylenediamine (EDA) gas in the presence of interfering substances such as ammonia and dimethylamine as well as benzene, toluene, xylene, and styrene gases, as measured using an asymmetric planar optical waveguide gas sensor. When the EDA gas molecule was adsorbed on the surface of the membrane, charge transfer between them preferably occurred, leading to a change in the membrane surface conformation. As an ideal sensing material for EDA gas detection, the PR-MOF-1 membrane showed a relatively high surface sensitivity (11,000 times cm-1) after 60 min of growth, and it could quickly (in less than 2 s) detect 1 ppb of EDA gas with a significant response (S/N = 3.45). During the static gas adsorption process, the EDA gas adsorption kinetics fit well with pseudo-second-order (PSO) model, and the adsorption capacity (qe) on a unit surface showed a high value of 33.91 μg cm-2 at 283 K. The high selectivity and sensitivity of the PR-MOF-1 membrane for EDA gas indicate the effectiveness of the light irradiation method for alteration of the metal- organic framework membrane structure and control of the gas sensing properties.Carbon nanomaterials are quite promising to be combined with metal-organic frameworks (MOFs) to enhance the sensing ability of both materials. In this work, a MOF nanoparticle of UiO-66-NH2 is integrated with carbon nanotubes (CNTs) (UiO-66-NH2/CNTs) with a facile solvothermal method. The morphology, surface area and properties of this UiO-66-NH2/CNTs nanocomposite was investigated using electron microscopy, XRD, XPS, BET analysis and electrochemical techniques. Catalytic oxidation of dopamine (DA) and acetaminophen (AC) on this nanocomposite was achieved, owing to a 3D hybrid structure or a large electroactive surface area, excellent electrical conductivity, a large number of active sites of this nanocomposite. It was further utilized as a sensing platform to establish an electrochemical sensor for the monitoring of both DA and AC. The enhanced oxidation signals led to the voltametric sensing of DA and AC in a broad linear range from 0.03 to 2.0 μM and low detection limits (S/N = 3) of 15 and 9 nM for DA and AC, respectively.