Additive manufacturing (AM) is today in the main focus-and not only in commercial production. Products with complex geometry can be built using various AM techniques, which include laser sintering of metal powder. Although the technique has been known for a quite long time, the impact of the morphology of individual powder particles on the process has not yet been adequately documented. This article presents a detailed microscopic analysis of virgin and reused powder particles of MS1 maraging steel. The metallographic observation was performed using a scanning electron microscope (SEM). The particle size of the individual powder particles was measured in the SEM and the particle surface morphology and its change in the reused powder were observed. Individual particles were analyzed in detail using an SEM with a focused ion beam (FIB) milling capability. The powder particles were gradually cut off in thin layers so that their internal structure, chemical element distribution, possible internal defects, and shape could be monitored. Elemental distribution and phase distribution were analyzed using EDS and EBSD, respectively. Our findings lead to a better understanding and prediction of defects in additive-manufactured products. This could be helpful not just in the AM field, but in any metal powder-based processes, such as metal injection molding, powder metallurgy, spray deposition processes, and others.Acoustic emission (AE) is a monitoring technique that has proven its suitability in and outside of the laboratory in characterizing the structural condition of materials. In composites for construction and repair, several breakthroughs have been recently noted involving mainly fracture mode evaluation based on the AE waveform characteristics. However, the acquired signals, apart from the cracking source strongly depend on the size and shape of the plate specimens. While the effect of wave propagation distance has been studied, the effect of the lateral dimension of the plate has not been given proper attention, being a broken link in translating the results from small coupons to real size plates. This paper examines wave propagation from artificial sources as well as actual AE signals in textile-reinforced cement (TRC) plates indicating the strong differences in the results that are attributed just to the shape and size of the specimens and showing that interpretation toward the actual sources is firmly connected to geometric factors.Cowpox virus (CPXV) belongs to the genus Orthopoxvirus in the Poxviridae family and is endemic in western Eurasia. Based on seroprevalence studies in different voles from continental Europe and UK, voles are suspected to be the major reservoir host. Recently, a CPXV was isolated from a bank vole (Myodes glareolus) in Germany that showed a high genetic similarity to another isolate originating from a Cotton-top tamarin (Saguinus oedipus). Here we characterize this first bank vole-derived CPXV isolate in comparison to the related tamarin-derived isolate. Both isolates grouped genetically within the provisionally called CPXV-like 3 clade. Previous phylogenetic analysis indicated that CPXV is polyphyletic and CPXV-like 3 clade represents probably a different species if categorized by the rules used for other orthopoxviruses. Experimental infection studies with bank voles, common voles (Microtus arvalis) and Wistar rats showed very clear differences. The bank vole isolate was avirulent in both common voles and Wistar rats with seroconversion seen only in the rats. In contrast, inoculated bank voles exhibited viral shedding and seroconversion for both tested CPXV isolates. In addition, bank voles infected with the tamarin-derived isolate experienced a marked weight loss. Our findings allow for the conclusion that CPXV isolates might differ in their replication capacity in different vole species and rats depending on their original host. Moreover, the results indicate host-specific differences concerning CPXV-specific virulence. Further experiments are needed to identify individual virulence and host factors involved in the susceptibility and outcome of CPXV-infections in the different reservoir hosts.Aeromonas veronii is a virulent fish pathogen that causes extensive economic losses in the aquaculture industry worldwide. In this study, a virulent strain of A. veronii TH0426 was used to establish an in vitro biofilm model. The results show that the biofilm-forming abilities of A. veronii TH0426 were similar in different media, peaking under conditions of 20 °C and pH 6. Selleck Shikonin Further, isobaric tags for relative and absolute quantitation (iTRAQ)-based quantitative proteomics methods were used to compare the differential expression of A. veronii between the biofilm and planktonic cells. The results show alterations in 277 proteins, with 130 being upregulated and 147 downregulated. Pathway analysis and GO (Gene Ontology) annotations indicated that these proteins are mainly involved in metabolic pathways and the biosynthesis of secondary metabolites and antibiotics. These proteins are the main factors affecting the adaptability of A. veronii to its external environment. MRM (multiple reaction 27 monitoring) and qPCR (qPCR) were used to verify the differential proteins of the selected A. veronii. This is the first report on the biofilm and planktonic cells of A. veronii, thus contributing to studying the infection and pathogenesis of A. veronii.Polyester fabrics were dyed with prepared disperse dyes using the high temperature dyeing method. The dye exhaustion of the dye baths were compared to the low-temperature dyeing method in an attempt to study the proportion of the dye effluent solution that affects the environment. The dye uptake of the high temperature dyeing method (HT) of polyester fabric was compared with low temperature dyeing, hence (HT) increased the color strength of the investigated dyes by 309 and 265%. This means that the amount of dye present in the dye effluents by using the high-temperature dyeing method is almost non-existent, and this is reflected positively on the environment as these wastes pollute the environment. Post-treated polyester fabric was prepared through a two-step hot process after being immersed in a solution of Titanium (IV) oxide nanoparticle sizeTiO2 NPs (21 nm primary particle size) at 80 °C followed by curing at 140 °C. The treated fabric realized an optimum UV protection factor of 34.9 and 283.6 degrees. These fabrics also demonstrated a strong ability to improve the light fastness properties.