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  • Martinsen Holman posted an update 1 day, 19 hours ago

    Cattle manure and poultry litter are widely used as fertilizers as they are excellent sources of nutrients; however, potential adverse environmental effects exist during land applications, due to the release of zoonotic bacteria and antimicrobial resistance (AMR) genes. This study was conducted to understand linkages between physiochemical composition, bacterial diversity, and AMR gene presence of cattle manure and poultry litter using quantitative polymerase chain reaction to enumerate four AMR genes (ermB, sulI, intlI, and blactx-m-32), Illumina sequencing of the 16 S region, and analysis of physical and chemical properties. Principal coordinate analysis of Bray-Curtis distance revealed distinct bacterial community structures between the two manure sources. Greater alpha diversity occurred in cattle manure compared to poultry litter (P less then 0.05). Redundancy analysis showed a strong relationship between manure physiochemical and composition and bacterial abundance, with positive relationships occurring among electrical conductivity and carbon/nitrogen, and negative associations for total solids and soluble fractions of heavy metals. Cattle manure exhibited greater abundance of macrolide (ermB) and sulfonamide (sulI) resistant genes. Consequently, fresh cattle manure applications may result in greater potential spread of AMR genes to the soil-water environment (relative to poultry litter) and novel best management strategies (such as composting) may reduce the release of AMR genes to the soil-water environment.Graphitic carbon nitride (g-C3N4), as the one of the most promising photocatalysts, usually relies on noble metal co-catalysts in the photocatalytic water splitting H2 evolution process, which greatly increases the use cost. Here, a zeolite imidazole framework (ZIF-67) derived Co@NC/g-C3N4 composite was constructed through facile thermal condensation of ZIF-67 and melamine. The obtained Co@NC/g-C3N4 composites can drive water splitting H2 evolution without any noble metal co-catalyst under simulated sunlight. The optimal sample exhibits the highest H2 evolution rate of 161 μmol g-1·h-1, which is 6 times of pure g-C3N4. The N doped carbon in carbonized ZIF-67 can not only quickly capture separated electrons from g-C3N4, but also serve as the co-catalyst. The well dispersed cobalt intermediate on carbonized ZIF-67 also play a role in promoting electron conversion. The formation of junction between carbonized ZIF-67 and g-C3N4 could promote quick charge carrier separation and transfer. This work provides a new idea for photocatalytic H2 evolution without noble metal co-catalysis.TAZ (transcriptional coactivator with PDZ-binding) zinc finger domains, also known as transcription adaptor putative zinc finger domains, that control diverse function in plant growth and development. Here, in the present study, we evaluated the role of the TAZ domain-containing gene in response to various heavy metals. Initially, we found a total of 3, 7, 8, 9, 9, 9, 7, 14, 6, 10, and 6 proteins containing TAZ domain in stiff brome, millet, sorghum, potato, pepper, maize, rice, apple, peach, pear, and tomato genome that could trigger the plant resistance against various heavy metals, respectively. Various in-silico approaches were applied such as duplication, phylogenetic analysis, and gene structure, to understand the basic features of the TAZ domain-containing genes in plants. Gene expression analyses were also performed under heavy metals (Cr, Zn, Ni, Cd, Co, Fe, Mn, and Pb). The results of quantitative real-time PCR analysis indicated that the TAZ gene family members were differentially expressed under different heavy metals. We further characterized the functions of the TAZ domain-containing gene under the heavy metal stresses by overexpressing the OsTAZ4 gene in Arabidopsis. The TAZ genes could promote plant resistance against various heavy metals by interacting with OsMYB34 and OsFHA9 transcription factors. D-Lin-MC3-DMA molecular weight The results will contribute to elucidate the relationship of TAZ proteins with heavy metals stresses and also ascertain the biological function in plant growth and development.Neonicotinoids sonochemical oxidation at high-frequency ultrasound (MHz range) has been carried out in ultrapure and natural surface-water matrices (river, reservoir and wastewater treatment plant effluent). To evaluate the influence of the operating variables, that is initial pollutant concentration, ultrasound frequency, ultrasound power, and pulse-stop time a Box-Behnken experimental design was planned. Optimal results were obtained using a frequency of 578 kHz, a power of 40 W L-1, with a pollutant concentration of 1 μM (for each pesticide), and using a pulse-stop time of 100 ms. The experimental data adjustment using the Langmuir-Hinshelwood heterogeneous kinetic model showed that neonicotinoids oxidation was carried out in the bubble-liquid interface by the attack of hydroxyl radicals. Experiments performed in the presence of radical scavengers, that is, methanol, ethanol and tert-butyl alcohol corroborated this reaction mechanism. The influence of some environmental conditions such as pH, presence of soluble inorganic species (Cl-, SO42-, NO3-, HPO42-, HCO3-) and soluble organic species (humic acids content) were established. Finally, the aqueous matrix’s influence was investigated for three natural surface water cases, and the results were rationalized according to the main water physicochemical characteristics.Waste water remediation is the ongoing hot research topic that can reduce the water scarcity all over the world. By reducing the pollutants in the waste water drawn from industries and other sources will be more useful for domestic purposes. To reduce the rate of pollutants in water may also help in improving the aquatic environment and decreases other side effects. Efficient and cost effective catalysts were in search for both dye degradation and water remediation treatment applications. NiMoO4 nanorods were prepared by employing co-precipitation method with different stirrer time (2 h, 4 h and 6 h). The formation of NiMoO4 was substantiated employing X-ray diffractometer analysis (XRD). Vibrational and rotational property of the samples was analyzed by FT-IR spectra and Raman spectra. The optical property was further confirmed by UV-vis spectral studies. Morphological analysis studies revealed growth of nanorods with 6 h stirrer time. The photocatalytic behavior of the obtained product was carried out under both UV light (364 nm) and visible light irradiation.

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