Western Blot was used to detect the protein contents of Ang II and Ang- (1-7) in liver tissue. Results The treatment group had less liver damage than the model group and the blocker group; BML-111 decreased the levels of ACE and AngII in serum of rats with CCL4 injury (P＜0.01) and increased the levels of ACE2 and Ang- (1 in serum -7) content (P＜0.01). BML-111 increased the content of Ang- (1-7) in liver tissue and decreased the content of AngII in CCL4 injured rat tissue. Conclusion The results showed that the intervention effect and mechanism of lipoxygen receptor agonist BML-111 on acute liver injury in rats may be related to the regulation of AngII and Ang-(1-7).Objective To investigate the effects of Sini San prescription(SNS) on the proliferation and apoptosis of HepG2 cells and its molecular mechanism. Methods The morphological changes of hepatocellular carcinoma HepG2 cells treated by SNS were observed by inverted microscope. MTT assay was used to detect the inhibitory effect of SNS on cell proliferation. Fluorescence staining and flow cytometry were employed to analyze the effect of SNS on apoptosis of HepG2 cells. Rho123 (Rhodamine 123) staining method was performed to detect the changes of mitochondrial membrane potential, and Western blot was used to evaluate the expression of proteins related to apoptosis. Results The number of hepatocellular carcinoma HepG2 cells were significantly decreased (P＜0.01) and cells showed typical apoptotic cell morphology after treated with serum contained SNS. The inhibition rate of HepG2 cells was increased with the increase of concentration of serum contained SNS. The number of cells in G1 phase was significantly increased, while G2 phase was decreased after treated with serum contained SNS(P＜0.05).The apoptosis rate and mitochondrial membrane potential of HepG2 cells were significantly increased and decreased after treated with serum contained SNS(P＜0.05). The expression levels of Bax, caspase-3,-9 and cyt-c were significantly increased, while the expression of bcl-2 was decreased in HepG2 cells treated with serum contained SNS(P＜0.05).Conclusion Sini San prescription can inhibit the proliferation of HepG2 cells and induce apoptosis by mitochondrial pathway.Objective To investigate the changes of nuclear factor erythroid 2-related factor 2 (Nrf2) and superoxide dismutase (SOD) in the prevention of insulin resistance in skeletal muscle of rats by aerobic exercise. Methods Twenty-four 12-month old Sprague-Dawley (SD) rats were randomly divided into control (C), insulin resistance model (IR) and insulin resistance model and exercise (IRE) groups. IR was induced by high- glucose and high-fat diet. Rats of IRE received the increasing load running on the treadmill for 6 W. The activity of total superoxide dismutase (T-SOD), catalase (CAT), Malonaldehyde (MDA) and glutathione / L-glutathione oxidized (GSH/GSSG) were measured by experiment kits. The content of 8-hydroxy-2 deoxyguanosine (8-OHdG) was measured by ELISA Kits. The expressions of Nrf2 and Glucose transporters 4 (GLUT4) in gastrocnemius were detected by Western Blot. Results ①Compared with IR, the content of HOMA-IR was decreased in IRE (P＜0.05), while the content of muscle glycogen was increased in IRE (P＜0.01). ② The activities of T-SOD, CAT, and GSH/GSSG in IRE were significantly increased compared with those in IR (P＜0.01). Compared with IR, the contents of 8-OHdG and MDA in IRE were significantly decreased (P＜0.01). ③ The expressions of Nrf2 and GLUT4 in gastrocnemius of IRE rats were increased than those in IR (P＜0.01). Conclusion The Nrf2 pathway was activated in gastrocnemius of rats after aerobic exercise, which promoted the activity of antioxidant enzymes, and prevented IR induced by high- glucose and high-fat diet.Objective To investigate the effects of 4-week electroacupuncture intervention on “Browning” of white fat in rats, and to explore its molecular mechanisms. Methods Twenty-four 8-week-old male SD rats were randomly divided into sedentary group (Sed), aerobic exercise group (Exe) and electroacupuncture group (ElA), 8 rats in each group. Exe group used 65% Max oxygen uptake intensity treadmill exercise, 1 h/d,6 d/w, while the ElA group used electric acupuncture to stimulate “zusanli” and “tianshu” points, 20 min/d,6 d/w, and the weight of rats was recorded every week. After 4 weeks of intervention, blood samples were collected from the apex and abdominal aorta. The wet weight of scapular fat and perirenal fat of rats was detected and the body fat and the serum levels of Irisin were determined. What’s more, the expressions of adenosine 5′-monophosphate activated protein kinase-α (AMPKα), phosphorylation of adenosine 5′-monophosphate activated protein kinase-α (p-AMPKα), peroxisome proliferator activated receptor 1). Conclusion 4 weeks of electroacupuncture intervention can effectively control the weight of rats and induce “browning” of white fat, and its effect was similar to aerobic exercise, which may be release Irisin through the AMPKα-PGC-1α-FNDC5-Irisin signaling pathway, and then “crosstalk” with adipose tissue, up-regulate the expression of UCP1, and induce “browning” of white adipose tissue.Objective To investigate the changes of metabolites of teenage football players after exercise-induced fatigue. Methods Twelve male teenage football players (14～16 yrs) were selected as experimental subjects in this study. And an exercise model including aerobic and anaerobic exercise as one group exercise was established by using power bicycle completion 6 min 150 W load, 60～65 r/min of riding exercise and 30 s of riding exercise which load was the maximum speed set by the tester’s weight. The rest took 1 min in the middle of one group exercise, and repeat 3 times of one group exercise, then rest for 3 min after one group exercise. The maximum oxygen uptake (VO2 max) and average anaerobic power were measured after each group exercise. Their urine samples were collected before and after the whole exercise model, and gas chromatograph-mass spectrometer (GC-MS) was used to detect the differential metabolites. Results The teenage football players had a significant decrease in anaerobic capacity after fatigue. selleck screening library Compared with pre-exercise, a total of 25 differential metabolites were screened out, of which 3 metabolites were significantly higher and 22 metabolites were markedly lower.