Background Exogenous application of gamma-aminobutyric acid (GABA) could relieve stress symptoms caused by abiotic stresses including anoxia, heat and cold shock, drought, and salt. However, studying translocation and metabolism of exogenous GABA is challenged by the presence of endogenous GABA. Results Herein, we used D6-GABA in order to investigate the uptake, translocation, and the metabolism of exogenous GABA in Mexican lime (Citrus aurantifolia) seedlings using gas chromatography-mass spectrometry (GC-MS). The GC-MS analysis showed that D6-GABA could be easily distinguished from the non-labeled GABA after methyl chloroformate (MCF) derivatization. The D6-GABA was detected in the cortex (phloem), inner stem (xylem), and leaves after root drench. Girdling did not affect the translocation of D6-GABA, indicating that it is mainly translocated via the xylem. In addition, D4-labled succinic acid was detected in D6-GABA-treated plants, indicating that exogenous GABA was metabolized to succinic acid. The half-life of D6-GABA in citrus was about 1.3 h, indicating a quick conversion to succinic acid. Conclusion The use of D6-GABA offers a valuable tool to study the translocation and metabolism of GABA in plants. D6-GABA and its metabolite (D4-succinic acid) can be easily distinguished from the endogenous GABA and succinic acid using GC-MS. © The Author(s) 2020.Background Image processing techniques have been widely used in the analysis of leaf characteristics. Earlier techniques for processing digital RGB color images of plant leaves had several drawbacks, such as inadequate de-noising, and adopting normal-probability statistical estimation models which have few parameters and limited applicability. Results We confirmed the skewness distribution characteristics of the red, green, blue and grayscale channels of the images of tobacco leaves. Twenty skewed-distribution parameters were computed including the mean, median, mode, skewness, and kurtosis. We used the mean parameter to establish a stepwise regression model that is similar to earlier models. Other models based on the median and the skewness parameters led to accurate RGB-based description and prediction, as well as better fitting of the SPAD value. More parameters improved the accuracy of RGB model description and prediction, and extended its application range. Indeed, the skewed-distribution parameters can describe changes of the leaf color depth and homogeneity. Conclusions The color histogram of the blade images follows a skewed distribution, whose parameters greatly enrich the RGB model and can describe changes in leaf color depth and homogeneity. © The Author(s) 2020.Background The relationship between anxiety and dietary fat quality (DFQ) has not been well studied. The aim of this study was to investigate the relationship between anxiety disorder and fatty acids’ intake in women. Methods This cross-sectional study included 300 women aged 18-49 attending healthcare centers. Dietary exposure was measured by a 168-item semi-quantitative food frequency questionnaire (FFQ). To determine the status of anxiety, the Depression, Anxiety, and Stress Scale (DASS) questionnaire was used. Based on the total score of anxiety, the participants were divided into two groups of without anxiety ( less then  8) and with anxiety (≥ 8). The relationship between fatty acids intake and odd ratio (OR) for anxiety was analyzed by simple logistic regression. Results About 37.7% of individuals reported anxiety. After adjustment for covariates, an increase in the OR for anxiety was observed across the quintuples of saturated fatty acids (SFAs) (OR 3.17; 95% CI 1.43-7.00; p-trend = 0.005). In addition, higher intakes of monounsaturated fatty acids (MUFAs) (OR 0.15; 95% CI 0.05-0.44; p-trend = 0.001), oleic acid (OR 0.25; 95% CI 0.09-0.67; p-trend = 0.002), alpha-linolenic acid (ALA) (OR 0.07; 95% CI 0.02-0.23; p-trend  less then  0.001), and n-3n-6 poly unsaturated fatty acids (PUFAs) (OR 0.56; 95% CI 0.24-1.03; p-trend = 0.02) were found to be related with lower OR of anxiety. Conclusion Intake of SFAs was positively related to anxiety disorder, whereas MUFAs, oleic acid, ALA, and n-3 n-6 PUFAs intake were inversely related to anxiety score. For investigating the association of fat intake and anxiety disorder, DFQ may be a useful measure. © The Author(s) 2020.Background Patients with depression who are treated with esketamine nasal spray may commonly experience dysgeusia (bad/metallic/bitter taste) and related side effects such as nausea and vomiting. While pretreatment with antiemetics can mitigate or prevent nausea and vomiting, it may not address dysgeusia as a contributing factor. Alternative interventions could help to manage vomiting due to dysgeusia following administration of esketamine nasal spray in those patients who are affected. Case presentation A 40-year-old man presented to the emergency department with depression and started treatment with an oral antidepressant. After providing informed consent to participate in a clinical trial evaluating the efficacy and safety of esketamine for major depressive disorder with active suicidal ideation with intent, he received 84 mg of esketamine nasal spray twice per week for 4 weeks. On the first 2 days of esketamine administration, the patient reported dysgeusia lasting several hours and intermittent retching lasting approximately 20 min. The patient was then given a fruit punch-flavored powdered drink (Crystal Light Fruit Punch™) approximately 25 min after nasal spray administration during the study period. The use of a fruit punch drink resulted in notable improvement of dysgeusia and associated vomiting, with time to resolution occurring within 30 min of the report of the adverse event. ABBV-744 cell line Conclusions A fruit punch-flavored powdered drink mix taken shortly after administration of esketamine nasal spray may rapidly manage and prevent vomiting due to dysgeusia. © The Author(s) 2020.Background About 76% and 85% of people in low and middle-income countries with severe mental illness did not get management because of fear of expected discrimination. Studying the intention to seek help for mental illness will, therefore, help to know their intended plan for help that would have a vital role to access patients with mental illness. Despite this, literature is limited in the area and community-based studies are scarce in Africa in general and Ethiopia in particular concerning help-seeking intention towards mental illness and its associated factors. Therefore, we assessed the pattern of intention to seek help and associated factors for mental illness among residents of Mertule Mariam town that would fill the gap in evidence and serve as baseline information for public health intervention. Methods A community-based cross-sectional study design was conducted from May to June 2017 at Mertule Mariam town. General Help-Seeking Questionnaire (GHQ) was used to assess the intention of help sought. Focus group discussion had also been employed to obtain qualitative data.