0 and 6.8). The results demonstrated that the adsorption ratios of 1.00-Al-Mt, 0.50-Fe-Mt and 1.00-Ti-Mt were 23.6%, 14.7% and 23.4%, respectively at pH 2.0 and 27.1%, 21.8%, and 27.4% correspondingly at pH 6.8 when added at 1.0 mg, which is 3-4 times higher than raw Mt (6.3-6.8% at pH 2.0 and 7.3-8.1% at pH 6.8). It was also found that with increased addition of pillared Mt (2.5 mg), the adsorption ratio approached 35%. The time for reaching equilibrium was approximately 120 min. These results demonstrated that Mt after pillaring modifications with Al, Fe and Ti can have potential for the control of DON in foods and feeds.Gluten protein as one of the plant resources is susceptible to genetic, physical, chemical, enzymatic and engineering modifications. Chemical modifications have myriad advantages over other treatments, including short reaction times, low cost, no requirement for specialized equipment, and highly clear modification effects. Therefore, chemical modification of gluten can be mainly conducted via acylation, glycosylation, phosphorylation, and deamidation. The present review investigated the impact of different chemical compounds on conformations of gluten and its subunits. Moreover, their effects on the physico-chemical, morphological, and rheological properties of gluten and their subunits were studied. This allows for the use of gluten for a variety of purposes in the food and non-food industry.Radish (Raphanus sativus) greens are commonly used as a vegetable in Korea; however, their anti-obesity effect has not been reported yet. We prepared the polysaccharide fraction of radish greens (PRG) and assessed its anti-obesity activity in high fat diet (HFD)-induced obese C57BL/6J mice. Supplementation with 4 mg/kg PRG reduced weight gain and body fat percentage, and regulated serum biomarkers against HFD-induced obesity. Moreover, PRG treatment improved gut permeability by increasing tight junction protein expression and colon length shortening. HFD intake increased the proportion of Firmicutes and decreased the proportion of Bacteroidetes and Verrucomicrobia; however, PRG supplementation maintained gut microbial composition to normal diet condition. Moreover, PRG reduced HFD-induced increase of lipid metabolism-related protein expression, along with adipocyte size in white adipose tissue. These results indicated that PRG as a potential prebiotic, has anti-obesity properties by improving gut barrier function, modulating gut microbiota and regulating lipid metabolism.In this study, the rheological and tribological properties of complex solutions comprising of fish gelatin (FG)-Arabic gum (AG), FG-xanthan gum (XG), and FG-κ-carrageenan (κC), respectively, were measured, as well as the effects of the complex on the physical properties of yoghurt. Results showed that with increased XG and κC concentrations, the viscosity of FG-XG and FG-κC complex solutions both increased. It was also found that the lubrication properties of FG-anionic polysaccharide (AP) solutions decreased with the increased AP contents. The applications of FG-AP complexes (FGAP = 91) improved firmness, water holding capacity and viscosity of yoghurt by the formation of large aggregates, but gels were easily destroyed at high frequency. Moreover, compared with gelatin, FG-AP complexes made yoghurt better lubrication properties during low and medium sliding speed, especially for FG-XG complexes. Thus, FG-AP complexes have the potential to be applied in producing yoghurt with good quality.Tadalafil (TDL) is an illegal additive drug found in drinks and functional foods that could threaten public health. There was a great concern whether the adulteration occurred in coffee added with similar type of herbs. Here we have developed a rapid, simple, sensitive, and semi-quantitative lateral flow immunoassay (LFIA) based on gold and fluorescence labelled monoclonal antibody (mAb) for detection of TDL in coffee sample. Under optimal conditions, the cut off limits using gold nanoparticles labelled mAb (GLM) was found to be 250 ng/mL and 100 ng mL using fluorescent labelled mAb (FLM) in coffee samples. The coffee samples were spiked with TDL, and the LFIA with GLM gave average recoveries of 92-105.3% (intra-assay) and 96.6-105.9% (inter-assay), meanwhile with FLM gave recoveries 97.9-107.3% (intra-assay) and 98.3-108.9% (inter-assay). Results gave LFIA with FLM more sensitive than with GLM and all the test can be completed within 10 min, which would be an option for convenient and rapid assay of TDL detection.The effect of thermal treatment of blueberry was investigated using a designed grinding and continuous packaging system under oxygen-free conditions. The grinding, packaging, and heating at 90 °C for 30 min under anaerobic condition were compared to heating under aerobic conditions, showing complete inactivation of oxidative enzymes. Heating without oxygen retained anthocyanins and ascorbic acid whereas heating in atmospheric air does not. Delphinidin glycoside was mostly influenced by oxygen deficiency during heating, followed by petunidin and malvidin glycosides. The differences in oxygen sensitivity may be closely associated with the number of hydroxylation in the B ring. The result of anthocyanin led to higher antioxidant activity and redness values of purees heated without oxygen than purees heated with oxygen. Consequently, thermal processing under oxygen-free condition can prevent oxidation of anthocyanin, resulting in higher retention of color and nutritional values of blueberry products.Medicinal mushrooms, Coriolus versicolor and Lentinus edodes are extremely attractive as nutraceuticals. Here we used fruiting bodies to prepare novel kombucha beverage. Microbiological, physicochemical and chemical properties were monitored for eleven days, while the immunological properties of kombucha polysaccharide extracts were determined in peripheral blood mononuclear cell (PBMC) cultures. FTIR analysis of polysaccharide extracts showed dominant presence of polysaccharides, in addition to phenols, lipids and proteins. C. versicolor kombucha extract displayed more complex polysaccharides, and a higher content of total polysaccharides, phenols and flavonoids compared to L. edodes kombucha extract. The extracts were not cytotoxic for PBMC in vitro up to 500 µg/ml, while immunomodulatory effects depended on their chemical compositions. The most prominent effect was on the reduction of Th2 cytokines and IL-10 in PBMC cultures. selleck compound Based on these results, novel kombucha products could be recommended as functional beverages or nutraceuticals with potentially beneficial immunomodulatory effects in allergies.